The nutrients, such as V A, V E, V C, amino acid, phospholipids, glucose oxidase DNA and RNA, in the pollen of Rosa Xanthina and Hemerocallis minor are studied and the result is that the nutrients especially of V A, V E, V C, EAA, DNA and RNA are rich and the proportion is good.
通过对黄刺莓和金针花粉的DNA ，RNA ，磷脂，葡萄糖氧化酶，氨基酸，VA，VE 和VC 等重要营养成分的测定，发现这两种花粉的营养成分丰富，配比良好，特别是VA，VE，VC 和必需氨基酸的质量比较高，RNA和DNA的质量比也较高。
By investigate the primary structure of strawberry ent-kaurene oxidase, we found that it has four conserve amino acid sequence.
对一级结构分析发现4段非常保守的区域，推测是在此酶行使功能时的重要组成部分。
Purpose To develop a new method to purify D-amino acid oxidase efficiently and fast.
目的 建立简便、快速纯化D-氨基酸氧化酶的新方法，以利用高纯度的DAAO转化头孢菌素C制备戊二酰－7-氨基头孢霉烷酸(GL-7ACA)。
The recent research of D-amino acid oxidase, including its distribution in the nature, physiological function, biological characteristic, catalytic mechanism, gene cloning, nucleotide sequence analysis and gene expression, are introduced in this paper.
介绍了D-氨基酸氧化酶的研究现状，包括在自然界的分布、生理功能、生物学特性、催化机理、基因克隆和序列分析以及基因表达。
The present investigation aimed to study the role of the D-amino acid oxidase in chiral inversion of D-NNA and the relationship between DAAO activities on various D-amino acids and their inversion rate.
研究了D型氨基酸氧化酶在D-NNA手性转化中的作用及DAAO对不同（包括已报道在体内可发生手型转化的）D型氨基酸的选择活性。
The simultaneous oxidation of cephalosporin C and glutaryl-7-aminocephalosporanic acid (Gl-7-ACA) by the hydrogen peroxide, which is formed as a transient product during the conversion reaction, is one of the key factors that result in the loss of the yield in the enzymatic conversion of CPC to Gl-7-ACA by D-amino acid oxidase.
在D-氨基酸氧化酶D1AAO转化头孢菌素C为戊二酰基－7-氨基头孢烯酸(Gl-7-ACA)的反应中，所生成的过氧化氢同时与CPC和Gl-7-ACA发生的氧化副反应是造成目标产物收率损失的关键因素之一。
Based on the techniques of genetic engineering and bioinformatics, the recombinant strains with a high-level expression of the D-amino acid oxidase and glutaryl-7- aminocephalosporanic acid (GL-7-ACA) acylase were constructed, and a new catalysis-separation coupling process was further developed for the production of 7-ACA in this dissertation.
为此，本论文从基因工程技术和生物信息学分析入手，成功构建了产D-氨基酸氧化酶和戊二酰基-7-氨基头孢烷酸（GL-7-ACA）酰化酶的高酶活重组菌，并在此基础上进一步发展了酶催化制备7-ACA的新工艺。
Interestingly, G72 protein interacts with the gene for D-amino acid oxidase on 12q24 to regulate glutaminergic signaling through the N-methyl-D-aspartate receptor pathway.
此外，嗜铬粒蛋白B基因由于其位于染色体上的区域，遗传研究的证据，以及其自身的功能，同样被认为是精神分裂症的候选基因。
In this study, the japonica rice cultivar Taiken 9 was transformed through an Agrobacterium-mediated method to express D-amino acid oxidase from Trigonopsis variabilis.
在此篇研究中，利用农杆菌基因转殖技术表现三角酵母菌的D-型胺基酸氧化酶於梗稻栽培种―台梗9号(Taiken 9)中。
AIM: To investigate the effect of purifried L-amino acid oxidase from bungarus fasciatus snake venom on apoptosis and growth of HUVCE cell line.
目的：探讨金环蛇毒中L-氨基酸氧化酶对人脐静脉内皮细胞株增殖和凋亡作用的影响。
Flow cytometry and laser confocal microscope showed a typical apoptotic peak and morphological changes of these cells. CONCLUSION: The L-amino acid oxidase from bungarus fasciatus snake venom could inhibit the HUEVC cell growth and induce the cell apoptosis.
通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，该氨基酸氧化酶是由单亚基组成的。L-氨基酸氧化酶能诱导HUVCE凋亡从而对其生长具有抑制作用。
The MTT assay was used to detect the viability of cells. Flow cytometry and laser confocal microscopy were used to identiyfy the cell cycle and apoptotic morphology after cells treated with LAO. RESULTS: An L-amino acid oxidase was successfully purified from the venom of bungarus fasciatus.
结果：通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，L－氨基酸氧化酶的表观分子量通过SDS-PAGE测定约为60 kD，通过凝胶过滤测定分子量为70 kD.L－氨基酸氧化酶作用于HUVCE细胞12 h后，细胞的生长明显受到抑制，L－氨基酸氧化酶作用的半数抑制量为2.8 mg/L。
The coding gene of a high-activity glucose oxidase mutant has the nucleotide sequence as shown in SEQ ID NO.1 and coded proteins have the amino acid sequence as shown in SEQ ID NO.2. The preparation method comprises the following steps: firstly, a pair of mutagenic primers is designed; secondly, a Escherichia coli strain DH5 alpha/GOD-A137S is obtained; thirdly, a recombinant plasmid pPIC9kGOD-A137S is identified; fourthly, the Escherichia coli strain DH5 alpha/GOD-A137S is cultured; fifthly, linear DNA containing mutant genes is obtained; sixthly, a yeast Pichia strain GS115/GOD-A137S is obtained; and seventhly, the target proteins are obtained after induction and purification.
这种高活力葡萄糖氧化酶突变体的编码基因具有SEQ ID NO.1所示的核苷酸序列，其编码的蛋白具有SEQ ID NO.2所示的氨基酸序列，其步骤是：首先设计一对诱变引物；第二是获得大肠杆菌菌株DH5α/GOD-A137S；第三是鉴定重组质粒pPIC9kGOD-A137S；第四是培养大肠杆菌菌株DH5α/GOD-A137S；第五是得到含突变基因的线性DNA；第六是获得毕赤酵母菌株GS115/GOD-A137S；第七是诱导纯化获得目的蛋白。
In the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of AOX. were synthesized by solid-phase method. The polypeptide was coupled to a -chymotrypsinogen, and the antibodies were obtained through immunizing rabbit by injecting this complex.
第一部分，我们按照交替氧化酶(Alternative Oxidase，AOX)位于线粒体内膜外侧亲水区S螺旋的氨基酸序列，用固相法合成由12个氨基酸残基组成的多肽，将此多肽与α-牛胰凝乳蛋白酶原A相连，用作半抗原，免疫家兔制备人工合成12肽的抗体。
METHODS: The L-amino acid oxidase was purified by SP-sepharose HP column followed by Heperin-Sepharose column.
通过阳离子交换层析和肝素亲和层析从金环蛇蛇毒中分离纯化到一个L-氨基酸氧化酶，SDS电泳鉴定其纯度。
The identification of protein were performed by searching the mass spectrometry data from the NCBInr database using the software of Sequest Bioworks3.2. The results indicated that over 25 protein components including acid and basis phospholipase A2, nerve growth factor, presynaptic neurotoxin, hemorrhagic factor, thrombin-like enzyme, serine protease, glycogen synthase, metalloproteinase, maturase, L-amino acid oxidase, plasminogen activator, adhesion, cysteine-rich secretory protein, disintegrin, fibronectin-binding protein, fibrinogen clotting inhibitor A chain, vascular apoptosis-inducing protein 1, pyranose 2-oxidase, carbamoyl-phosphate synthase, as well as the homology proteins of snake venom ablomin, ablomin, catrin, mucrofirase, pallabin, salmosin and triflin homologues were identified from the GSSV.
从GSSV中鉴定出超过25种蛋白组分，主要包括：酸性磷脂酶A1、碱性磷脂酶A2、神经生长因子、突触前神经毒素、出血因子、类凝血酶、丝氨酸蛋白酶、糖原合酶、金属蛋白酶、成熟酶、L-氨基酸氧化酶、纤溶酶源激活物、外源凝集素、富含半胱氨酸分泌酶、去整合素、纤连蛋白结合蛋白、纤维蛋白原凝固抑制因子A、血管凋亡诱导蛋白1、氨甲酰基磷酸合成酶、吡喃糖－2-氧化酶以及ablomin，catrin，mucrofirase，pallabin，salmosin和triflin的同源蛋白。
Aloe Vera contains: Amino Acids - alanine, arginine, asparagine, aspartic acid, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, valine. Enzymes - alinase, amylase, bradykinase, carboxypeptidase, catalase, cellulase, glucose oxidase, lipase, malic dehydrogenase, protease. Minerals - aluminium, calcium, copper, iron, magnesium, manganese, potassium, sodium, zinc. Vitamins - A, B1, B2, C, E, Choline, Folic Acid, Inositol, Niacin.
芦荟成份包含：丙胺酸、精氨酸、天冬酰胺酸、天门冬氨酸、谷氨酸、谷酰胺、氨基乙酸、组氨酸、异亮氨酸、白氨酸、赖氨酸、苯基丙氨酸、脯氨酸、丝氨酸、苏氨酸、酪氨酸、缬氨酸、淀粉酶、羧肽酶、接触酵素、钙、铜、铁、镁、锰、钾、钠、锌、VA、VB1、VB2、VC、VE、复合VB、叶酸、纤维糖、烟酸。
Studies of Amino Acid Oxidase Constructed by β - Cyclodextrin Derivant
β-环糊精构筑氨基酸氧化酶的研究
Recent Research Progress of D - Amino Acid Oxidase
D-氨基酸氧化酶的研究进展
Immobilization of D - amino Acid Oxidase from T. variabilis
三角酵母D-氨基酸氧化酶的固定化研究