Background: Yersinia pestis is the etiologic agent of bubonic and pneumonic plague, which has claimed numerous lives in human history.
背景：鼠疫耶尔森氏菌是烈性传染病鼠疫的病原体，曾给人类带来了巨大的灾难。
The aim of this research is to find out the experimental parameters that affecting the results when analyzing bacteria by MALDI TOF MS, and to set up a general sample preparation protocol for establishing mass database of Bacillus anthracis and Yersinia pestis. It will play an important role in rapid identification of bacteria, and tracing its origin in the scenario of bioterrorism and outbreak of infectious disease for microbial forensic analysis. Methods:As for the universalness of sample preparation protocol for bacterial analysis by MALDI TOF MS, bacteria being investigated included: gram-positive bacteria including B.
本研究通过对MALDI TOF MS分析细菌的方法进行系统的研究，阐明影响MALDI分析的重要实验因素的作用，并建立MALDI TOF MS分析细菌的标准实验方法，从而实现不同菌种和同一种内不同菌株的鉴定，为建立炭疽芽孢杆菌和鼠疫耶尔森氏菌的质量图谱数据库奠定基础，从而能够快速准确地检测和鉴定我国不同来源的炭疽芽孢杆菌和鼠疫耶尔森氏菌，在生物恐怖或传染性疾病爆发时，作为微生物法医学的一种重要技术，快速、准确地鉴定细菌，并追踪其来源。
Ann G. Carmichael, MD, PhD, maintains that the bubonic bacillus, Yersinia pestis, was most certainly a factor in the outbreak of plague, but, at the same time, the fearsome reputation of the Black Death may have hidden or deemphasized the emergence of other interacting epidemics such as smallpox, influenza, anthrax, typhus, meningococcemia, mycosis, and acute diarrheal diseases.
医学博士安妮·G·喀米舍尔认为，耶尔森氏菌最有可能是瘟疫爆发的一个因素，但是黑死病令人胆颤的大名可能使得其他一些相互影响的传染病被人们所忽视，比如天花、流行性感冒、炭疽、斑疹伤寒症、脑膜炎球菌血症、真菌病以及急性腹泻等等。
Results The testing results showed that this method with 100% specificity and sensitivity in 300 positive and 100 negative samples of mammalian sera respectively. However, the positive serum samples of Francisella tularensis, yersinia pseudotuberculosis and yersinia enterocolitis could not be deceted by dipstick of ICA.
结果 间接血凝法检测阳性300份血清标本，胶体金法检测全部为阳性，间接血凝法检测阴性100份血清标本，胶体金法检测全部为阴性，两种方法符合率100％；并且鼠疫胶体金法快速诊断盒不与假结核耶尔森氏菌等相关细菌的免疫血清发生交叉反应。
To analysis bacterial-host infection, an in vitro experiment was first carried out to analyze the acid responses of Yersinia pseudotuberculosis, a gastro-intestinal bacterium, under acidic condition. Metabonomic variation of Y.
到21d时，天冬酰胺和组氨酸浓度降低，而缬氨酸、乳酸、丙氨酸、苹果酸、琥珀酸、GABA、胆碱、β-葡萄糖、果糖、尿囊素、苯丙氨酸、甲酸的含量增高。
Objective Investigation was precced in rodent's Yesinia in plague focus and non-plague focus fo Guangdong, to understand the circumstance that the distrbution of Yersinla, and whether the restrained phenomenon of yersinia was existed. Meth-ods IHA and RIP was used for surveying the particular FI Ab of yersinia pestis. Yersinia pestis were surveyed by plague culture me-dia. The contents of ileocecal part, the liver, the spleen and the tongue of rodent's were put into PBS that were propagated（4℃）for3weeks, then the above PBS liquid was smeared on selective culture media to sarvey yersina enterocolitica and Yersina pseudotuberculosis etc.
目的 了解广东省鼠疫疫源地和非鼠疫疫源地鼠类耶尔森氏菌的分布和相互抑制的情况方法分别采用间接血球凝集试验、放射免疫沉淀试验进行鼠疫耶尔森氏菌特异性F1抗体检测，并分别对鼠类回盲部内容物和肝、脾、舌进行冷增菌后，以选择培养基分离。
Meth-ods iha and rip was used for surveying the particular fi ab of yersinia pestis. yersinia pestis were surveyed by plague culture me-dia. the contents of ileocecal part, the liver, the spleen and the tongue of rodent's were put into pbs that were propagated（4℃）for3weeks, then the above pbs liquid was smeared on selective culture media to sarvey yersina enterocolitica and yersina pseudotuberculosis etc. yersinia.
分别采用间接血球凝集试验、放射免疫沉淀试验进行鼠疫耶尔森氏菌特异性f1抗体检测，并分别对鼠类回盲部内容物和肝、脾、舌进行冷增菌后，以选择培养基分离。
Objective: Studying on the character of IS 1541 of Yersinia pestis.
目的：研究鼠疫耶尔森氏菌的插入序列IS 1541的插入位点的特性。
The distribution of IS 1541 in Yersinia pestis was different. It can be a gene marker and used in gene typing
IS 1541在鼠疫菌中的分布有差异，可以作为该菌的基因标志，用于分型。
Bacteria Yersinia pestis were sensitivity to 22 of 26 antibiotics.
鼠疫菌对26种抗菌药物中的22种敏感。
There are 168bp in band 2 with a homologv of 84% with the Yersinia pestis strain.
这两个片段的结构和功能还有待于进一步的研究。
Objective Establishing and developing methods for quick, special and sensitive detection of Yersinia pestis.
目的发展一种快速、敏感、特异的检测鼠疫耶尔森菌方法。
Cheopis is responsible for transmitting the bacterium Yersinia pestis, the causative agent of plague.
十印负责转递鼠疫细菌，病原体的瘟疫。
ResuLts indicate that ten species of pathogenic bacteria(Staphylococcus aureus, Clostridium botuLinum, Clostridium perfringens, Shigella sonnei, Vibrio cholerae, Proteus vuLgaris, Vibrio mimicus, Listeria monocytogenes, Yersinia enterocolitica, Bacillus cereus) show high sensitivity and specificity for the oligonucleotide array; Vibrio parahaemolyticus showed low sensitivity for the oligonucleotide array; Vibrio fluvialis gave weak cross-reaction with Vibrio parahaemolyticus; Streptococcus hemolytic-βgave weak cross-reaction with Listeria monocytogenes and Bacillus cereus; but the reaction did not affect their detection.
结果表明10株食源性致病菌(金黄色葡萄球菌、肉毒梭状芽孢杆菌、产气荚膜梭菌、宋内氏志贺氏菌、霍乱弧菌、普通变形杆菌、拟态弧菌、单核增生李斯特菌、小肠结肠炎耶尔森氏菌、腊样芽孢杆菌)的检测具有高敏感性和特异性；副溶血性弧菌的敏感性相对较低；河流弧菌同副溶血性弧菌存在很弱的错杂交，乙型溶血性链球菌的检测同单核增生李斯特菌和腊样芽孢杆菌存在很弱的错杂交，但是均不影响检测结果。
Objective To clone and express the important pathogenic genes in chromosome of Yersinia pestis in E. coli.
目的 在大肠杆菌中表达鼠疫耶尔森氏菌染色体编码的重要致病相关基因。
Objective: To establish an Anti-contamination multiplex PCR microplate hybridization enzyme immunoassay for identifying Yersinia pestis.
目的：建立鼠疫耶尔森氏菌防污染多重PCR—微孔板杂交—EIA检测鉴定技术用于鼠疫耶尔森氏菌的快速检测鉴定。
Methods The colloidal gold labeled with protein A of S taphylococcus aureus, contected with the polysaccharide antigen of Yersinia pestis to pyroxylin membrane and assembled them to the dipstick of ICA.
采用柠檬酸三钠还原法制备胶体金颗粒，标记金黄色葡萄球菌A蛋白作为探针，同时用鼠疫多糖抗原致敏硝酸纤维素膜，共同组装成基于免疫层析原理的胶体金法快速诊断盒。
The second worldwide plague pandemic occurred in 1346~1351, the plague destroyed almost the whole of Europe. In 1347 by the parasites in the mice of a flea carrying the plague bacillus Yersinia of the Mediterranean ports spread through Sicily, and in 1350 spread throughout the European continent.
第二次世界范围内的鼠疫大流行发生在1346年~1351年，这场鼠疫差一点毁灭了整个欧洲。1347年，由寄生在老鼠身上的跳蚤携带的耶尔森氏鼠疫杆菌经地中海各港口传到西西里岛，到1350年传遍了整个欧洲大陆。
To facilitate the functional analysis of chromosomal genes and their products in Yersinia pestis, we adapted the recombineering technique to epitope tagging of chromosomal genes of Y.
为加快研究鼠疫菌染色体基因及其产物的功能，我们将重组工程技术应用于标记鼠疫菌染色体基因。
A pair of primers derived from this fragment can specifically amplify Yersinia pestis templates.
根据这段序列设计的引物对可以特异性地扩增鼠疫耶尔森菌的模板。