Methods Microparticle enzyme immunoassay and enzyme linked immunosorbent assay were adopted to detect all indicators of hepatitis B (HBsAg, HBsAb, HBeAg, HBeAb, HBcAb, Pre-Sl protein), Hepatitis C virus antibody, HIV antibody, Treponema pallidum antibodies of13on blood patients in Renmin Hospital of Wuhan University during January-December 2008. The operation strictly obeyed the method and evaluation criteria of kit.
用微粒子酶免分析法与双感染抗原夹心酶联免疫法对武汉大学人民医院2008年l-12月13072例接受输血治疗的患者检测乙型肝炎全套指标（HBsAg、HBsAb、HBeAg、HBeAb、HBcAb、前Sl蛋白）、丙型肝炎病毒抗体、艾滋病病毒抗体、梅毒螺旋体抗体，严格按照试剂盒的操作方法与评判标准进行。
However, the patient developed similar episode of pain, redness and poor vision in the same eye after 3 weeks. Ocular examination revealed severe anterior uveitis with presence of mutton fat keratic precipitates. Venereal disease research laboratory and treponema pallidum hemagglutination assay were positive suggesting syphilis. The condition improved markedly with benzathine penicillin injection and topical steroid.
然而，术后3wk，右眼再次发生疼痛、红肿、视物模糊，眼部检查显示严重的前葡萄膜炎伴有羊脂状角膜后沉积物出现，VDRL和TPHA阳性证实为梅毒，通过全身注射青霉素类药物和局部应用类固醇药物，病情明显好转。
On exposure source analysis, 51.89% and 16.98% were patients with positive HBsAg and antibody of treponema pallidum, 2 patients with positive anti-HIV.
外科医生发生锐器伤的比率最高，达67.44%，暴露多发生在医生使用缝合针、穿刺针或刀片进行手术时。
Objective: To explore three kinds of method for detecting treponema pallidum.
目的：探讨三种血清梅毒螺旋体检测方法的应用价值。
The positive rate of Treponema pallidum antibody in patients with malignant tumor is high.
恶性肿瘤患者梅毒感染的阳性率较高。
It's a good way to detect treponema pallidum with ELISA and PPPA method.
ELISA法和TPPA法在临床的诊断中是较好的血清梅毒螺旋体的检测方法。
It's a good way to detect treponema pallidum with ELISA and PPPA method. clin-lab. com
ELISA法和TPPA法在临床的诊断中是较好的血清梅毒螺旋体的检测方法。
Objective To study the necessity of the screening and confirmation of Treponema pallidum infection by three serologic tests.
目的 探讨3种梅毒螺旋体血清学试验用于梅毒螺旋体感染的初筛和确认的必要性。
Methods ELISA and TPPA were used to measure Treponema pallidum antibody in 5, 324 inpatients with malignant tumor.
用ELISA法和TPPA法对在我院住院并确诊为恶性肿瘤的5324例患者进行梅毒螺旋体抗体检测。
Objective To explore the positive distribution of Treponema pallidum antibody in patients with malignant tumor, and to analyze its distribution in different tumor diseases.
目的 探讨恶性肿瘤患者梅毒TP感染的阳性分布趋势及其在不同肿瘤疾病中的分布情况。
Objective To compare characteristics of treponema pallidum antibody screening method with the toluidine red unheated serum test, the enzyme-linked immunosorbent assay and the T.
目的比较无偿献血者梅毒抗体筛查方法甲苯胺红不加热血清试验、双抗原夹心法的酶联免疫吸附试验和梅毒螺旋体明胶凝集试验的特性。
Methods 34933 serum samples were screened by ELISA method on Treponema pallidum antibody. The samples with probable positive result were retested by toluidine red unheated serum test and Treponema pallidum particle assay. Results There were 559 serum samples with probable positive result.
对34933例标本采用酶联免疫吸附试验进行梅毒螺旋体的初筛，初筛结果为可疑阳性的样本进行梅毒螺旋体明胶凝集试验和甲苯胺红不加热血清反应素试验的检测。
4075 patients being accepted intraocular operation from 2002 were confirmed by preoperative serological tests of these four infectious diseases including HbsAg, HCV Antibody, TP Antibody, and HIV Antibody. Infection ratio of syphilis in intraocular operational patients were analysed. The specific and unspecific antibody in blood and aqueous humor of syphilis patients was detected by rapid plasma regain circle card and treponema pallidum gem agglutination assay.
对2004年以来准备接受内眼手术的4075例患者进行常规的传染四项检查，包括乙肝表面抗原，丙型肝炎抗体，梅毒抗体，艾滋病抗体四项指标，并统计梅毒发生的阳性率；应用特异性抗体试验一梅毒螺旋体血球凝结试验和非特异性抗体试验一快速血浆反应素环状卡片试验检测梅毒患者血清及房水中的抗体。
Methods Three serologic methods including domestic-made enzyme-linked immunosorbent assay reagents, the toluidine red unheated-serum test and treponema pallidum particle agglutination test reagents were compared. The transudate fluid of the syphilis patient in genital duct was detected by polymerase chain reaction for analysis of infectivity.
采用国产酶联免疫吸附试验试剂与甲苯胺红不加热血清试验和梅毒螺旋体颗粒凝集试验进行血清学方法比较，采用聚合酶链反应对梅毒患者生殖道皮损渗出液进行检测，分析其传染性。
Methods To detect the antibody of Treponema pallidum in 182 serum specimens with RPR、TPHA、TPPA and ELISA methods.
摘 要］目的：评价在梅毒早期诊断中，四种梅毒血清学检测方法的比较及在临床诊断中应用价值。
Analysis: by FQ-PCR, darkfield microscopy for Treponema pallidum, and serologic testing for syphilis.
本研究表明FQ-PCR检测生殖器溃疡TP在一期梅毒诊断中有一定的价值。
Treponema pallidum, all antigens, Rabbit anti -, Biotin.
梅毒螺旋体，全抗原，兔抗，生物素。
ObjectiveTo assess the application value of fluorescence quantitative polymerase chain reaction for detection of treponema pallidum DNA.
目的：评估荧光定量聚合酶链反应技术检测梅毒螺旋体DNA的应用价值。
In order to correctly diagnose hard chancre, physicians are expected to generally analyze the case history, clinical features, laboratory examinations including biopsy and dark field examination for Treponema pallidum.
诊断硬下疳仍然需要对患者的病史、临床表现、实验室检查，包括活检以及暗视野检查梅毒螺旋体作综合分析。
MethodUsing FQ-PCR technique to detect treponema pallidum DNA in hard chancre, syphilitic bubo, the lesions of secondary syphilis (flat condyloma, macula, maculopapule, papule, node, mucous patches), the whole blood of patients with syphilis, and TP-DNA positive samples preserved in sterile tube at room temperature for 1～3 and a half years as well as some pollution goods.
使用FQ-PCR技术检测硬下疳、一期横痃、二期梅毒皮损、梅毒患者全血、室温保存1～3年半的梅毒螺旋体DNA阳性标本以及部分生活污染品中的梅毒螺旋体DNA含量。