Immunohistofluorescent staining was used to identify the binding specificity of GEBP11 in gastric cancer tissues. 5. GEBP11 was labeled with ~(99)Tc~mO_4~- using direct labeling method, and was labeled with ~(131)I using NBS labeling method. Then the labeled peptides were validated for radiochemical yield, specific activity, and in vitro. stability. 6. The bioactivity of ~(99)Tc~m-GEBP11 or ~(131)I-GEBP11 was validated by cell receptor autoradiography in cultured HUVECs. 7. Receptor binding assay was performed in vitro. to analyze quantitatively the binding specificity and affinity of GEBP11 to HUVEC, the receptor intensity on HUVEC, and the difference of affinity of GEBP11 binding to Co-HUVEC and HUVEC. 8. The radioactivity of all organs of nude mice injected with ~(131)I-GEBP11 was determined and the radioactivity of all organs per g was calculated to analyze the specific distribution of ~(131)I-GEBP11.9. Gamma camera images of nude mice bearing tumor xenografts of human gastric carcinoma injected with ~(99)Tc~m-GEBP11 was obtained 0.5-24 h after being injected to identify the targeting ability of labeled peptide to tumor tissues. 10. MTT assay on HUVECs and antitumor assay in nude mice bearing tumor xenografts of human gastric cancer were performed to evaluate the effects of ~(131)I-GEBP11 on HUVECs and xenografts. 11. Immunohistochemical staining was used for MVD counting in tumor. 12. H. E. staining, blood cells analysis and blood biochemical indicator analysis were performed to investigate the injury of liver and bone marrow. 13. Tube formation assay in matrigel, CAM angiogenesis assay and angionenesis induced by matrigel in mice were performed to identify the effects of GEBP11 on angiogenesis.
1、通过蓝色噬斑形成实验及GEBP11、IN11噬菌体在荷瘤裸鼠体内的竞争抑制实验检测噬菌体体内归巢的特异性；2、免疫荧光技术检测GEBP11在荷瘤裸鼠体内的结合特异性；3、免疫细胞化学或荧光检测GEBP11在Co-HUVECs中的定位及内化；4、免疫组织荧光检测GEBP11在人胃癌组织中的结合特异性；5、采用直接法~(99)Tc~mO_4~-标记GEBP11，采用NBS法~(131)I标记GEBP11，纸层析法测定标记率、放射化学纯度等；6、放射自显影鉴定GEBP11同位素探针的结合活性；7、受体放射配基结合分析实验鉴定受体亲和力及受体细胞密度；8、同位素示踪技术检测~(131)I-GEBP11在荷瘤裸鼠体内的生物学分布；9、SPECT成像技术进行荷人胃癌裸鼠体内~(99)Tc~m-GEBP11显像；10、MTT细胞增殖实验、荷瘤鼠抑瘤实验评价~(131)I-GEBP11对内皮细胞及荷瘤鼠肿瘤的抑制能力；11、免疫组织化学染色计数瘤组织MVD；12、病理学HE染色、血液血细胞分析及生化指标检测观察肝脏损伤及骨髓抑制情况；13、Matrigel管状结构形成实验、鸡胚绒毛尿囊膜血管生成抑制实验、小鼠体内Matrigel Plug诱导血管生成实验分析GEBP11对血管生成的影响；14、MTT细胞增殖实验、细胞周期及凋亡分析、细胞侵袭迁移实验、细胞粘附实验探讨GEBP11抑制血管生成的细胞机制；15、基因表达谱芯片技术筛选GEBP11短肽作用Co-HUVECs后的差异表达基因。
Aiming at above problems, the objective of this experiment is to discuss correlation between indices of SPECT liver ASGP receptor scintiscan with the ligand of 99mTc - ASF and classical liver functional index, and sensitivity of those indices.
针对以上问题，本研究试图以99mTc—ASF作为配体探讨SPECT肝脏ASGP受体扫描的有关指标与常规肝功能指标间的相关性，并比较各项指标的灵敏性。
In recent years, the quantitation of ASGP receptor by 99mTc-labeling neogalactoalbumin SPECT scintiscan is the up-to-date method that is applied to quantitatively evaluate hepatic function reserve.
以99mTc标记去唾液酸糖蛋白作为特异性配体，通过SPECT闪烁扫描测定肝脏去唾液酸糖蛋白受体的量，以此评估肝脏储备功能是近年来开展的定量评估肝脏储备功能的新方法。
Results: Until 2005, the allocation volume of CT was 164, and MRI was 95, DSA...
结果：截至2005，北京市拥有164台CT、95台MRI、78台DSA、39台SPECT、25台LA。
At present vari- ous countries in the world are carrying out an extensive studies in this re- spect.
目前世界各国正在积极地研究它，可是影响胶粘剂寿命的因素很多。
During ictal SPECT studies, 18 showed hyper-perfusion in solitary lobe, multi-hyper-perfusion foci in 10 patients. Compared the two examinations, 26(86.7%) were accorded with each other in 30 patients. Of the 12 patients with multiple and diffuse hypometabolism in interictal PET, 6 patients showed foci changed to solitary focal hyper-perfusion in ictal SPECT.
发作期SPECT显像18例为单叶局限性高灌注，10例为多处高灌注灶。30例患者中26例(86.7%)两项检查结果相符合，12例PET发作间期呈多病灶或弥漫性改变者，6例于发作期转化为单叶局限性病灶。
A new sort of fiber optic sensor was designed, the variation of refractive i ndex of resin surrounding the embedded fiber optic was mean to observed by measu ring the speckle spatial spect rum at the end-face of the fiber optic.
本文报道了一种新型光纤传感器，其原理为通过测量光纤末端近场模斑谱来反映埋置在周围的树脂折射率的变化。
Among the 221 patients with CTPA results, the abnormal segments detected by CPTA were 976, and by planar pulmonary imaging 796; the coincident rate was 81.6%.
在221例有CTPA诊断结果的患者中，CTPA显示受累肺段为976个，平面肺灌注显像显示796个肺段阳性，两者符合率为81.6％；SPECT肺灌注显像显示937个肺段阳性，SPECT肺灌注显像与CTPA的符合率为96％；两符合率之间差异有统计学意义（P〈0.01）。
Results: Thirty articles including 14MG (2941 lesions), 10 MRI (1428 lesions), 17 PET/SPECT (2247 lesions) met the inclusion criteria. The pooled sensitivity mean(95%CI of US, MG, MRI and PET/SPECT were 82%(76-86%), 86%(83-88%), and 87%(83-90%) respectively, pooled specificity, 69%(62-75%), 65%(62-69%)and 82%(76-86%).
结果：30篇文献(部分为比较影像学研究涉及两个或两个以上检测手段)，14篇关于MG(2941个病灶)，10篇关于MRI(1428个病灶)，17篇关于PET/SPECT(2247个病灶)，符合纳入标准。
Besides in 5 patients with tumor central necrosis, 99mTc-tetrofosmin SPECT showed central activity defect comfirmed by operation.
有5例手术证实伴有肿瘤中心坏死的肺癌病灶，其99mTc-TF SPECT显像示肿瘤中心部位放射性缺损。
Clinical application of 18 F-FDG SPECT/CT coincidence imaging in patients with nasopharyngeal carcinoma.
如果您喜欢本文请加入收藏夹，谢谢。本论文发布日期：2008年07月24日本论文作者：刘生，许泽清，梁
17 patients with a single subcortical lesion were examined with regional cerebral blood flow 99mTc-SPECT imaging.
对17名单一皮层下梗死病灶的卒中患者进行99mTc-SPECT脑血流灌注显象。
With the aid of a hydrogen lamp, illuminating at 5~8 X 10-8 Sec., the flight time of photoelectrons of serveral kinds of photomultiplier has been determined by employing the oscilloscopic method; an investigation as to the effect of the outer circuit of RCA-5819 for resolution time has been made and the better working state suitable for instant observation for the spect...
在发光时间为5～8×10~(-8)秒的氢灯帮助下，用示波方法对几种光电倍增管的光电子飞行时间进行了测定；研究了RCA-5819外电路对分辨时间的影响，从而确定较佳工作状态，使其适用于火花光谱激发过程作瞬时观测之用。
We also found that plasmid vectors combinding with PEI reagent, then directly transfected into mice bearing HepG2 hepatoma, cannot produce good gene expression imaging by microPET, planar and optical imaging due to low transfection efficiency. We have constructed adeno-associated viral vector with HSV1TK reporter gene triggered by tissue-specific enhancer and promoter such as EIIA in combination with AFP, tumor-specific promoter hTERT and nonspecific promoter CMV. It showed that AAV vector contribute to higher transfection efficiency to tumors but low toxicity and long-term gene expression in tumor cells, consequently producing better gene expression image in hepatoma by using FIAU probes and SPECT even at 7 days after injection.
我们同时也发现利用分子聚合物PEI转染试剂混和质体载体从尾静脉做活体直接转染的转染效率较低，无法於microPET、2D planar与optical imaging上产生良好的基因表现影像；建构的AAV腺卫星病毒载体具有特异性与非特异性的促进子与启动子，具特异性的部分如EIIA促进子与甲型胚胎蛋白启动子的组合、hTERT启动子，非特异性的部分则以CMV启动子为代表。
18F-FDG myocardial metabolism imaging and late contrast-enhanced MRI were employed to detect viable myocardium, and the accuracy of MRI was analyzed.
应用双核素SPECT存活心肌显像及心脏MRI延迟增强显像分析术前左室存活心肌，比较分析MRI检测的准确性。
Objective To provide an accurate and scientific method of radionuclide imaging for the clinical application and to study the value of SPECT imaging in detection and localization of the lesions in diagnosing pyelonephritis and renal scars in children, we evaluated the sensitivitiy and specificity of planar and planar+SPECT imaging with 99 Tcm-DMSA renal scan in diagnosing the pyelonephritis and renal scars in an experimental piglet model of pyelonephritis and renal scars established in this hospital.
目的 制作猪肾盂肾炎和肾脏瘢痕动物模型，评价99Tcm-二巯基丁二酸肾脏平面显像和SPECT断层显像诊断肾盂肾炎和肾脏瘢痕的灵敏度和特异性，并探讨SPECT的诊断价值。
This thesis mainly introduces the design of digital SPECT forepart detector.
论文主要介绍数字化SPECT探头部分的设计。
Another kind of imaging reagent of radioactive 3-quinuclidinyl 4-iodobenzilate was also studied in this paper. Iodine-123 was the ideal isotopes of radioiodine for SPECT (Single Photon Emission Computed Tomography) imaging and was elected to label peptides and other compounds, but for it is expensive and not easy to obtain in our lab, iodine-125 was substituted to study the labeling method and primary biodistribution.
碘-123以其合适的γ射线能量（159KeV，83％）而成为SPECT的理想选择，但由于碘-123主要通过加速器来制备，价格昂贵，不易得到，因此在方法学研究和初步的动物实验中，我们使用碘-125来代替碘-123，并建立起一套完整、可靠的方法，为碘-123标记奠定基础。
RESULTS:After remote regional myocardial infarction, the parameters of STI did not significantly change; the total notching number in HFECG increased markedly within 2～4 weeks and recovered at 8th week; the radial rarefaction and defect were indicated in infarction area of anterior wall of myocardium with 2～4 weeks in SPECT, and these abnormalities ameliorated obviously at 8th week.
HFECG的切迹数在2～4周时明显增多，而在8周时恢复；在2～4周时，SPECT显示心肌前壁梗死区呈放射性稀疏及缺损，而在8周时，该异常明显恢复。
Methods 32patients with lung cancer, 4patients with pancreatic cancer, 7patients with hepatocarcinoma and2patients with ovarian cancer were collected thoracal and abdominal coincidence imaging with2ual-head coinciˉdence SPECT after fasting more than8h and Venojection296MBq 18 F-FDG.
32例肺癌、4例胰腺癌、7例肝癌和2例卵巢癌病人，禁食8h以上，静脉注射296MBq18F-FDG分别于45min后显像，采集胸、腹部符合线路图像。