Methods In this study, a plasmid vector, rAd5, and rAAV2 were used to transfect rat cochlear marginal cells of stria vascularis by injection into the perilymph through the round window membrane, and the transfection efficiency, target tissue accessibility, cell/ tissue toxicity, time course of expression, and effect on hearing were evaluated in vivo and in vitro.
结果三种载体对原代培养的大鼠耳边缘细胞的转染研究发现，腺病毒的转染效率最高，而腺相关病毒转染对细胞活性影响最小。rAAV2和rAd5对大鼠耳蜗组织的感染实验发现，腺病毒和腺相关病毒携带的增强型绿色荧光蛋白可在多种耳蜗组织中及转染对侧耳蜗组织表达，且并不引起明显的耳蜗组织细胞凋亡。rAAV2携带的EGFP基因在第90天时仍可检测到大量表达。rAd5携带的EGFP基因在第30天时表达明显减弱。
We had a really rad time in Florida during spring break.
2在春假中我们在佛罗里达玩的非常开心。
Gene therapy is expected to yield a rad ical cure of mycobacterial tubercul...
借助于各种先进的医疗设备而进行精确的诊断，基因治疗将成为一种崭新的治疗手段。
Production and sales companies: particle detector strength, freeze-drying machine, snow ice machine, glass reactor, rotary evaporator, electronic balance, ultrasonic cleaning machines, ice making machine, industrial ice-making machine, constant temperature and humidity box, microscope several significant oil bath temperature pot/water bath pot, Ma fluoride furnace, low temperature cooling liquid circulating pump, circulating water-type multi-purpose vacuum pump, mold incubator, biochemical incubator, constant temperature incubator, vacuum oven, blast oven, super - net workbench, biosafety cabinets, magnetic stirrer, centrifuge, ultrasonic cell pulverizer, ultra-pure water machines, autoclaves, laboratory various beaker, Bio-Rad power supply, test-bed, constant temperature circulator, low temperature cycle at high pump, pesticide Tachometer, UV analyzers, liquid machine, PCR device, liquid chromatography, weather chromatograph, total acidity/PH, the spectrophotometer, ultra-low temperature refrigerators, constant-current pumps, rotary vane type vacuum pump, auto parts traps, low-temperature thermostat bath; and other products.
公司生产销售：颗粒强度测定仪，冷冻干燥机，雪花制冰机，玻璃反应釜，旋转蒸发器，电子天平，超声波清洗机，冰块制冰机，工业制冰机，恒温恒湿箱，显微镜，数显恒温油浴锅/水浴锅，马氟炉，低温冷却液循环泵，循环水式多用真空泵，霉菌培养箱，生化培养箱，恒温培养箱，真空干燥箱，鼓风干燥箱，超净工作台，生物安全柜，磁力搅拌器，离心机，超声波细胞粉碎机，超纯水机，高压灭菌器，实验室各种烧杯，电泳仪电源，实验台，恒温循环器，低温循环高空泵，农残速测仪，紫外分析仪，移液枪，PCR仪，液相色谱，气象色谱仪，酸度计/PH计，分光光度计，超低温冰箱，恒流泵，旋片式真空泵，自动部分收集器，低温恒温槽；等系列产品。
One day a Rad Hen found a green seed.
一天，一只红母鸡发现了一粒绿色的种子。
Yes, we do got one angle, rad, by'Math. acos', but in radians.
是的，我们通过 cos 得到了一个角度
I have a dream, that one day, on the rad hill of J##, the son of formal slaves and the son of formal slaveowners, will seat together...
of brotherhood 我高中背过的，现在只记得这么多了，不过很感动的
Countless people enter game world of programming since writing the Russian square game, Because this since one examine RAD good method of developing instrument, Examine a person to develop language, environment, basic datum structure knowledge skilled convenient way of degree too.
无数人进入游戏编程的世界都是从编写俄罗斯方块游戏开始的，因为这是检验一个人对开发语言、环境和基本数据结构知识熟练程度的便捷途径。
The beam emittance of the storage ring is 2.33 nm*rad, that is about one third of the existing light source of similar energy in the world.
光源储存环的束流发射度达到2.33 nm*rad，是目前世界上现有的同样能量的光源储存环的发射度的三分之一左右。
And what strategies will Shanghai RAD apply on sailing and marketing?
方根公司又采取了哪些与众不同的销售及品牌策略？
Results The titers of rAd reached 1010 PFU/ml and more than 95%B16-F10 cells could be infected by 20 MOIs rAd.
目的探讨含hB7-1基因的重组腺病毒hCD80rAd对B16-F10肿瘤细胞体外生物学特性的影响。
In budding yeast, both pathways depend on the RAD6–RAD18 post-replication repair pathway and are largely controlled through covalent modifications of proliferating cell nuclear antigen (PCNA; an essential processivity clamp for DNA polymerases) by ubiquitin37, 39BOX 2; FIG.
在出芽酵母中，两条途径都依赖于RAD6–RAD18复制后修复途径，并且主要由泛素通过对增殖细胞核抗原(PCNA，DNA聚合酶必需的具有持续合成能力的滑行夹)的共价修饰来调控(BOX2；图4)。
Also, the influence to the tracking velocity and tracking precision of the system brought by the different size of laser beam, the relative location relationship between the laser beam and the detector, and the laser intensity distribution are discussed.
对于极窄的激光束(10-6~ 10-3 rad量级)和极远的传输距离(如地球至同步轨道上航天器的通信距离可达40 000 km以上)，要实现稳定的对准十分困难，这需要由专门的空间光束捕获、对准、跟踪技术和系统来完成[1]。
There are some apple on the tree, and all of the apples are rad.
树上有一些苹果，所有的苹果都是红的。
It would be rad if that picked up.
这将是拉德如果上升。
And if you hit the game with a mi'rad and it wounds it, you can eat.
如果你击中球，加上mi'rad ，而且伤口的话，你可以吃。
The new is amp is going to be rad.
新的音箱要发售了。
Take right circular polysilicon thin film flexure hinge as an example, the rotational stiffness calculated using the macro theory is 8 N-μm/rad and the tested result is 120 N·μm/rad.
宏理论计算和微机械测试结果之间的偏差较大，这表明在微尺度效应的影响下，宏机械柔性铰链理论模型不能直接用于薄膜型硅微机械柔性铰链的计算。
By use of experimental data, the new quality map and the popular one are compared and the unwrapping results are obtained.
最后通过实验数据，比较新质量图和相位导数偏差质量图在解包裹相位算法中的作用，得到新质量图的解相结果均方根值为2.652 rad，而相位导数偏差质量图的解相结果均方根值为5.151 rad，由此证明前者有更好的可靠性。
Objective To evaluate the inhibitory effect of recombinant adenovirus rAd-MK-TNFα on cell growth of breast cancer in vitro.
目的 初步观察重组腺病毒rAd-MK-TNFα对乳腺癌细胞的抑制作用。