RT-PCR were carried out for PC tissue specimen to validate proteins ident...
结果：1。获得了重复性好、分辨率高的胰液双向电泳图谱。2。
The highest level of expression was observed in the stem and leaf tissues and no obvious signal was detected in the root and coleoptile tissues.
通过RT-PCR和对Rht近等基因系的抑制缩减杂交，我们还克隆了14个有明显开放读码框的EST序列。
The FABP gene cDNA fragment was amplified by RT-PCR and cloned into pMD19-T vector for sequencing and analyzing.
FABP基因cDNA亚克隆到pcDNA3.1中，构建核酸疫苗pcDNA3.1-FABP-NM，经测序验证，结果正确。
Specific primers were designed according to published nucleotide sequence of FABP gene in the GenBank database.
摘要 根据GenBank中细粒棘球蚴脂肪酸结合蛋白基因cDNA序列设计引物，并在起始密码子前加上Kozak 序列，提取细粒棘球蚴的原头蚴总RNA，RT-PCR扩增目的基因。
In all kinds of matching condition, the lengths of stimulus onset asynchrony have no affects to the RT.
在各种匹配条件下，SOA的长短均没有影响被试的RT。
RT-PCR analysis showed that the gene omk1 transcribed in the phases of both spore germination and hyphal growth.
该基因的克隆将为进一步研究该菌识别寄主信号的分子机理奠定基础。
Methods MTT was used to measure the levels of the proliferation of U251 cultured with FC at different concentrations.
方法用MTT比色法检测不同浓度FC对U251细胞的增殖抑制，免疫组织化学和RT。
In addition to the capacity of transmission SCYLV between sugarcane plants efficiently, 100%(9/9), 75%(6/8), and 50%(4/8) respectively while 15 viruliferous aphids were inoculated for each plant.
建立了能够检出单头蚜虫及侵染早期未现症植株体内SCYLV的巢式RT-PCR技术，在超过80%的饲毒2周以上的甘蔗绵蚜无翅成虫体内检测到病毒的存在。
It was carried out at 60℃ for 50min, and its sensitivity was 10-1 TCID50, which was 100 times more than the traditional RT-PCR method.
该方法在60℃下保温50min即可完成，可检测至10-1 TCID50的病毒，与常规RT-PCR检测方法相比灵敏度高100倍。
TCID50 BVDV could be detected by RT-PCR, and 10-1 TCID50 BVDV could be detected by nested PCR.
该技术检测到病毒最低浓度达10~(-1)TCID_(50)水平。
Indeed Skyjack abandoned its venerable differential drive system on its 68RT line to make an RT scissors with 32 feet of platform height, for example, retract down to a 69-inch stowed height.
商业建筑的诱惑力非常大，以致于大多数主要剪式升降机制造商- Genie、Haulotte、MEC和Skyjack都在过去的18个月里引入了新型RT剪式升降机。
Objective: To establish a method of real-time fluorescent RT-PCR and routine RT-PCR for detecting Norovirus.
目的：建立快速、特异的诺如病毒实时荧光RT-PCR与常规RT-PCR相结合检测方法，对人感染诺如病毒进行快速检测。
In order to examine the role of astacene on mice body development and the expression of energy metabolism related genes in mice, we treated mice and primary culture of mouse muscle cells with astacene of higher and lower concentration. Then the total mRNA was extracted from the muscle tissue and cells respectively, and the mRNA levels of UCP3 and LXRα were detected by RT-PCR in all the samples.
本试验用高、低浓度虾红素日粮饲喂昆白系小鼠和处理原代培养小鼠骨骼肌细胞，提取总RNA，检测各时段UCP3、LXRa基因mRNA表达量，探讨虾红素对小鼠个体发育、肌肉能量代谢相关基因表达变化规律的影响。
In this dissertation, the molecular characteristics of acetylcholinesterases, carboxylesterase, and nicotinic acetylcholine receptor in L. bostrychophila, L. entomophila, and L. decolor were investigated. The project is supported by National Natural Science Foundation of China(30570231) and the Program for New Century Excellent Talents in University(NCET-04-0854).The major results are summarized as follows:1 Gene cloning of esterases and mRNA expression level in L. bostrychophila1.1 Gene cloning and sequence analysis of AChE in L. bostrychophilaThe second full length cDNA encoding AChE(GenBank Accession No.:FJ647185) was cloned from L. bosrtychophila by the methods of reverse transcriptase PCR and rapid amplification of cDNA ends. The complete cDNA of this gene consists of 3316 bp with an open reading frame of 2814 bp, encoding a protein of 937 amino acids residues with the putative signal peptide of 23 residues. The mature protein has a molecular weight of 104.8 kDa with an isoelectric point of 6.63.This gene is paralogous to AChE of Drosophila melanogaster by sequence homology analysis, and thus named Lb acel.
本研究在国家自然科学基金(30570231)和教育部新世纪优秀人才支持计划(NCET-04-0854)的资助下，重点研究了嗜卷书虱、嗜虫书虱和无色书虱酯酶(包括乙酰胆碱酯酶和羧酸酯酶)及乙酰胆碱受体的分子生物学特性，获得了以下主要研究结果：1嗜卷书虱酯酶基因克隆及其mRNA表达水平研究1.1嗜卷书虱乙酰胆碱酯酶基因克隆及序列分析采用RT-PCR和RACE技术成功克隆获得了嗜卷书虱第2个乙酰胆碱酯酶基因的全长序列(GenBank登录号：FJ647185)，该基因全长3316bp，开放阅读框2814 bp，编码一个937个氨基酸组成的AChE前体蛋白，其中包括了由23个氨基酸组成的信号肽，成熟蛋白的分子量为104.8 kDa，理论等电点为6.63。
When compared with control spinal cord samples taken from non-injected animals, ipsilateral expressions of 5-HT〓 receptor mRNA 1 h or 4 h after bee venom injection significantly increased by 80.94% or 37.86%, respectively. There were little changes in 5-HT〓 receptor mRNA level on the contralateral side at these two time points.
本课题联合应用分子生物学方法（半定量RT-PCR、Western Blot）、药理学方法和行为学方法（皮下注射蜜蜂毒诱发炎性痛的行为学观察），研究了脊髓背角内5-HT〓受体在伤害性信息传递过程中的作用。
Stupp R, Mason WP, van den Bent MJ, et al: Concomitant and adjuvant temozolomide and radiotherapy for newly diagnosed glioblastoma multiforme: conclusive results of a randomized phase III trial by the EORTC Brain and RT Groups and YCIC Clinical Trials Group.
星形细胞瘤则为5～10年。毛细胞性星形细胞瘤则为正常寿命。许多脑膜瘤[1]和垂体腺瘤[2]的病人一直没有症状。这些肿瘤被偶然发现，或在死于其它疾病后尸检发现肿瘤。
We detected the level of transcription by real-time quantitative RT-PCR analysis.
转染效率通过荧光定量RT-PCR标定，使其保持一致。
Objective: To evaluate the efficacy and safety of recombinant tissue plasminogen activator and to explore the most suitable dosage of rt PA in the early treatment of the Chinese patients with acute cerebral infarc tion.
目的 ：评价重组组织型纤溶酶原激活剂艾通立对急性脑梗死早期静脉溶栓治疗的疗效及安全性，同时探讨国人应用 rt PA治疗的最佳剂量。
The fusing gene was transfer into the mammary gland tissue of mice and cows.
RT-PCR法克隆目的基因，通过酶切、连接、分离、纯化等方法构建含tPA-cDAN的乳腺定位表达载体；采用乳腺注射法将融合基因转入小鼠及牛的乳腺组织中。
Finally, the results of the real-time network system and the modularized software architecture for power conversion systems are implemented.
最后，论文分别对RT-PES-Net和模块化软件方案进行了相应的实验研究和分析。