According to the results of gene localization, we have isolated the BamHI-PstI fragment of ETS of rRNA gene of silkworm Attacus ricini from a subclone pAR II.
根据rRNA杂交定位的结果，分离了带有外转录间隙区的蓖麻蚕rRNA基因次级克隆 pARⅡ的BamHI-PstⅠ片段。
The frequency and characteristics of SSR derived from ESTs by sequencing complementary DNA library of PST were analyzed.
本研究首次对小麦条锈菌cDNA文库蕴含的EST-SSR进行了的分析，明确了小麦条锈菌EST-SSR的基本特征。
The minimum accumulation occurred at 96 h, at which transcripts were only 0.07 fold of that in uredinisopore. During incompatible interaction between Pst and wheat, PsCdc2 was down-regulated and its accumulation was lower than that in urediniospore.
在非亲和组合中，该基因表达基本呈下调趋势，在接种后各个时间点的表达量均低于在夏孢子中的表达量，其中接种后12 h时表达量最高，但仅为夏孢子中表达量的0.34倍；接种后96 h表达量最低，为夏孢子中表达量的0.02倍。
Real time RT-PCR analysis showed that in compatible interaction between Pst and wheat, PsCdc2 was up-regulated at early stage of infection. The maximum induction occurred at 12 hpi, at which transcripts were 1.62 fold over that in urediniospore. From 24 to 268 hpi, the accumulation of transcripts decreased steadily.
实时荧光定量RT-PCR结果表明，在亲和组合中，该基因在条锈菌接种小麦的前期上调表达，其中接种后12 h时表达量最高，约为夏孢子中表达量的1.62倍，接种后24-268 h，基因表达基本呈下调趋势，其中96 h基因表达量最低，仅为夏孢子时期的0.07倍。
Image taken using ATK-1HS II webcam on Coronado PST-Ha and 2x barlow lens.
使用 ATK-1HS II 摄像头配合Coronado PST-Ha 和两倍巴罗镜。
The PST of stray light includes the influence of system assemble error, and reflects the real capacity of stray light suppression.
光学系统杂光指标PST的测量，包含了系统各种安装误差的影响，体现了系统杂光抑制的真实水平。
For Ontario, we collect PST on all goods that are taxable under the province's tax base.
根据省的税制，在安大略省，我们对所有应税货物收取
For British Columbia, we collect PST on all goods that are taxable under the province's tax base.
根据省的税制，在B.C省，我们将收取所有货物的
It is known the mechanism is Pto kinase intereaction with any of the two effectors of the bacteria, then signal to the downstream component active the resistance responses in this disease system.
这一模式识别的分子机制是Pto激酶与PST的两个效应子AvrPto和AvrPtoB中的任一个发生物理互作，然后与Prf一起激活下游多重信号传导途径，进而产生抗病性反应。
A new type of difunctional initiator (benzyl dibromide, ethyl dibromoacetate) was proposed in this paper and used to synthesize poly based triblock copolymers such as PSt-PBA-PSt, PMMA-PBA-PMMA.
本论文提出一种新型ATRP双官能团引发剂(如α，α-二溴化苄、α，α-二溴乙酸乙酯)，并用聚合反应速度法、GPC法、核磁分析法进行双官能度的验证，并通过两步聚合合成以聚丙烯酸酯为中间嵌段、苯乙烯或甲基丙烯酸甲酯为两端嵌段的ABA型三嵌段共聚物。
The highly sophisticated Eagle e-bike energized by TranzX PST utilizes a proprietary lithium polymer power pack and features a series of innovative components.
高度复杂的 Eagle 电动车采用 TranzX PST 动力系统并使用独特的锂聚合物动力组件，并配备一系列创新的零件组成。
In compatible interaction of Pst and wheat, PsCon1 was up-regulated as early as 6h post-inoculation.
PsCon1在小麦条锈菌萌发夏孢子时期基因表达量为新鲜夏孢子中的1.69倍。
Earth will pass through one of the denser debris streams at around 4 am EST (1 am PST) Tuesday.
地球将穿过4时左右在一个密集的碎片流美东时间上午（1早上8：30）星期二。
Then, the comparative study on PST and range segment method is given, which shows that PST has better performance on strong clutter discretes rejection because it exploits more information inherent in the data.
并对分段方法中的最优的分段数目、段内的样本选取方式以及对消形式等问题进行了研究。
Methods With a standard papillotome(transpancreatic duct pre-cut), a plough-like papillotome or a needle-knife papillotome, immediate PST was carried out in 67 patients recently when conventional method was not able to visualize the biliary tract or deep cannulations proves difficult during ERCP.
方法67例患者在实施ERCP诊疗操作中，当常规方法不能使胆系显影或胆道深部插管困难时，即用标准刀、犁状刀或针状刀行乳头预切开，观察预切开的效果和近期并发症。
Continuous growth of dendrons from G1.5 to G4 at the both ends of PSt chains was carried out by the iterative Michael addition reactions with excess PZ and following TT. Structures of the triblock polymers were characterized by GPC and ~1H NMR spectra.
随后，聚合物链末端反复用过量的哌嗪和TT进行迈克尔加成反应，使树枝状聚酰胺胺嵌段不断增长，得到G1.5到G4的嵌段共聚物。
The effects of polymerization reaction parameters such as medium polarity, concentration of St-P4VP macromonomer and polymerization temperature on the sizes and size distribution of P4VP-g-PSt microspheres were investigated.
同时探讨了多种聚合反应条件（介质的极性、St-P4VP大分子单体的浓度和反应温度）对微球粒径与粒径分布的影响。
Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.
首先在DNA2.0和Gene2Oliga软件辅助下对lipA基因密码子及RNA二级结构进行优化并引入Cla I(237位)和Pst I(475位)酶切位点；通过组装PCR分别合成lipA基因的各片段F1(237 bp)、F2(238 bp)和F3(422 bp)；通过该基因内的Cla I和Pst I限制性酶切位点连接成完整的全长lipA基因。
The results indicated that the protoplasts of the fresh Pst urediniospores just collected from wheat leaves shrank into many small dollops under the hydrochloric acid of 24.5% to 36.5%.
结果表明，24.5%、28.5%、32.5%、36.5%4种浓度的盐酸处理条锈菌夏孢子后其原生质体均浓缩成多个分散的小团。
A variety of experiments were conducted to reviewed the impact factors for the performance of PSt-acrylamide magnetic particles, including the dosage of Fe_3O_4 magnetic cores and acrylamide, whether to use divinylbenzene or not.
通过一系列实验考察了Fe_3O_4磁核的用量、丙烯酰胺的用量以及是否使用二乙烯基苯等的变化对生成的聚苯乙烯丙烯酰胺磁性颗粒性能的影响。