Then, sections were exposed to either fluorescein Texas Red labeled secondary antibody and visualization was commenced by means of laser fluorescence microscopy.
将20例人前列腺移行带组织用甲醛固定后制作成冰冻切片（10μm），先应用PDE5抗体进行孵化，然后应用Texas Red将标本进行标识，用荧光显微镜观察结果。
Methods Twenty cases cryostat sections (10 μm) of formaldehyde-fixated tissue segments excised from the transition zone of human prostates were incubated with primary antibody directed against the PDE isoenzyme 5. Then, sections were exposed to either fluorescein Texas Red labeled secondary antibody and visualization was commenced by means of laser fluorescence microscopy.
将20例人前列腺移行带组织用甲醛固定后制作成冰冻切片(10 μm)，先应用PDE5抗体进行孵化，然后应用Texas Red将标本进行标识，用荧光显微镜观察结果。
Data replotted according to procedure of Dixon plots. Results Three PDE isoenzyme peaks were purificated from bovine corpus cavernosum.
以不同剂量的PDE5选择性抑制剂西地那非作用于PDE5，经Dixon Plots法处理结果以观察西地那非的抑制效应。
The research results show that a PDE surface can avoid the construction of continuous functions and a compound PDE surface reconstruction can conveniently realize the crosssectional contour surface continuity of the first order or more parameters.
实验结果表明，PDE重构断层间轮廓线表面避免了连接函数的构造，而且基于组合PDE曲面的断层间表面重构方法能方便地实现轮廓线间表面的一阶或一阶以上的参数连续。
According to the theory of PDE, the closed form solution of the model is obtained.
利用偏微分方程理论，求出偏微分方程的解析解。
Phosphodiesterases is one of phosphodiesterases families that are particularly abundant in smooth muscle and blood platelet.
磷酸二酯酶v(pde5)是存在于平滑肌、血小板等组织中一种有特定结构和功能的磷酸二酯酶亚型。
The study items include The influence of the rectangular fin surface along the height air flows on heat-transfer coefficient, Simulation of temperature field using three-dimensional finite element analysis by commercial software PDE Toolbox, Comparison of the fin`s temperature distribution measured and simulated, Optimal design regarding fin thickness to fin efficiency The best combinations for fin thickness and height, Analysis of optimal fin-to-fin spacing.
主要之研究项目为：探讨发射单体之矩形鳍片之散热面沿气流流动的高度对於对流系数的影响，利用PDE Toolbox分析软体进行鳍片三维有限元素分析模拟温度场，实验量测鳍片之温度分布与模拟结果之比较，探讨鳍片厚度对散热鳍片效率影响，探讨鳍片厚度与高度之最佳设计关系，鳍片最佳间距分析。
Pulse detonation engine is a new concept of propulsive device, which use high temperature and pressure gas produced by intermittent or pulsed detonation wave to generate thrust. PDE has many desirable advantages, including high thermodynamics cycles efficiency, simple configurations, and large range of usage, etc. so various research plans about PDE have been laid and carried out.
脉冲爆震发动机是一种利用间歇式或脉沖式爆震波产生的高温、高压燃气来产生推力的新概念发动机，这种发动机具有很高的热循环效率、结构简单、适用范围宽广等优点，很多国家都已经将其列入重点发展的先进推进系统研究计划之中，开展了大量研究。
Pulse detonation engine is a new concept of propulsive engine which uses high temperature and pressure gas produced by intermittent or pulsant detonation wave to generate thrust. PDE has many desirable advantages, including high thermal dynamic cycle efficiency, low specific fuel consumption, high thrust/weight and specific impulse, simple configuration, and will become propulsive device of aviation and spaceflight aerocraft in 21st century.
脉冲爆震发动机是一种利用间歇式或脉冲式爆震波产生的高温、高压燃气来产生推力的新概念发动机，这种发动机具有循环热效率高、燃料消耗率低、推重比高、比冲大、结构简单等优点，将成为21世纪新型的航空航天飞行器的动力装置。
And 77.44% of that caused by Xoo wi Id type 13751 at high inoculation concentration, respectively, and was 14.47%, 77.99% and 76.10% of that caused by 13751 at low inoculation concentration, respectively, indicating that XoopdeA, rpfG and pcaD genes are required for the full virulence of Xoo on r i ce. The analysis of Xoo PdeA revealed that its ami no acid 317-488 formed DUF1 domain with c-di-GMP phosphodiesterase A activity and ami no acid 498-745 formed DUF2 domain with diguanylate cyclase activity.
平板检测表明xo口p丈介绒一突变体GXNI 256、rP招一突变体GXNI 258的胞外多糖和OSF因子的产量均显著减少，说明xo口胞外多糖和OSF因子的产生与rP招和p尤论斌基因有关。xo口pde月基因产物的功能域分析表明其第317一488个氨基酸为具有环二鸟昔酸磷酸二醋酶A活性的OUFI域和第498一745个氨基酸为具有二鸟昔酸环化酶活性的DU「2域，这两个酶可能参与调控细胞内信号分子环二鸟昔酸的浓度。xo口rP凡基因产物的功能域分析表明第1 92一329个氨基酸为HD域，属于依赖金属的磷酸水解酶家族。
Tadalafil is a long-acting PDE-5 inhibitor, which is effective for up to 36 hr in the majority of men.
达拉是长效了PDE5抑制剂，是有效时限为36小时，在大多数的男人。
It is suggested that a decrease in intracellular cAMP is a possible mechanism for CCh to modulate I_; During the EDS and IDS CCh controls the cytoplasmic cAMP level by different pathways: In EDS, CCh modulates I_ possibly by activating PDE which accelerates the breakdown of cAMP, but in IDS possibly by inhibiting adenylate cyclase which then reduces the synthesis of cAMP.
因此，我们推断，CCh可能是通过调控细胞内的cAMP水平来调节I_的。
Methods: Non-injury stress stimulus method similar to human spirit stress was used to extablish the Ganyu Qizhi animal model, and the PDE5 activity in corpora cavernosa of the rat penis was measured by the method of immunohistochemistry and computer image analysis.
采用与人类精神性应激十分相似的非损伤性应激刺激法，制造肝郁气滞型SD大鼠动物模型 4 0只，随机法将大鼠分为：空白组；肝郁证造模组；萎康凝胶高剂量给药组和低剂量给药组 4组，每组 10只。以免疫组化与计算机图像分析技术测定海绵体组织中PDE5活性。结果：高剂量组阴茎海绵体组织中PDE5活性与模型组差异有显著性(P 。0 1)。
When we apply Fourier transformations to the whole- and half-line PDEs, it is necessary to perform the inverse Fourier transformations to derive the PDE solutions, and it is quite often that those integrals involve the square root operator which is multi-valued in the complex plane.
当我们运用傅立叶转换解整个或半平面的偏微分方程时，需要利用逆傅立叶转换导出该偏微分方程的解，此时被积分函数中常出现平方根的形式，在复数平面上它是多值函数。
Results show that simple structure aerovalves can be applied in PDE because it has both functions of check valve and air-blast atomizer.
结果表明，结构简单的气动阀具有单向阀和气动雾化喷嘴的功能，能够用于PDE。
After that, we use similarity reduction to get a simple PDE with only one space variable and its boundary conditions. And we use the finite difference method to solve it.
再利用相似解化简方法，对此定价模型进行降维处理，并导出边界条件，进而使用有限差分法求出方程的数值解。
Crossflow drying is one of the methods widely used in grain drying. Three mathematical models for crossflow drying by researchers of USA were introduced in detail, including Bakker-Arkema PDE model, two-dimensional dynamic PDE model, DP process model. Model predictive control method and its application in continuous crossflow grain dryer were also presented in this paper. All the introductions were aimed at providing some references for the similar research in our country.
横流干燥是目前应用较为广泛的粮食干燥形式之一，该文详细介绍了美国学者建立的适用于谷物横流干燥的Bakker-Arkema偏微分方程模型、二维动态偏微分方程模型、分布式参数DP过程模型；并简要介绍了模型预测控制方法及其在连续式横流谷物干燥机上的研究与应用，以期为中国的同类研究提供借鉴和参考。
To explore the function of PDE in canine central nerve, gene expression of 18 PDE subtypes in different fractions of canine central nerve (cerebrum, cerebellum, myelencephalon and spinal cord) were detected by RT-PCR, and PDE activity was calculated by the content changes of cyclic nucleotides before and after the PDE reaction examined by high performance liquid chromatography.
为探明磷酸二酯酶(phosphodiesterase，PDE)在犬中枢神经活动中的作用，采用反转录聚合酶链反应检测18个PDE亚型在犬中枢神经不同部位中(大脑、小脑、延髓和脊髓各段)的表达分布，以高效液相色谱法检测环核苷酸在酶反应前后的含量变化，计算PDE活性。
Sidenafil and other PDE5Is have been approved for the treatment of erectile dysfunction or primary arterial hypertension.
西地那非等PDE5抑制剂已被用于勃起功能障碍和肺动脉高压的治疗。
The above results suggest indirectly that the immunoregu- lation of MT may be related to its affecting AC activity and not be related to PDE activity.
深入研究发现，MT的这一作用可被百日咳毒素（PT，1mg·〓）所取消。表明MT抑制淋巴细胞AC活性、降低cAMP水平是通过激活Gi蛋白实现的。