ABSTRACT: Random amplified polymorphic DNA was used in analysing the relationship among twenty-six speices of Amanita from Mangshan, Hunan. Of 40 primers, 6 ones generated reproducible RAPD profiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species of Amanita by its RAPD profiles. The similarity coefficients obtained from profiles generated by six primers among Amanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clustering analysis. It indicated that there were two groups in 26 species and some species with ring and membranous volva clustered a group which coincided with morphological classification.
本文对采自湖南莽山的26种鹅膏菌属真菌进行了随机扩增多态性DNA分析，40个随机引物中筛选出扩增效果较好的6个引物，每个引物能产生1～10条DNA条带，获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来，通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20―60%之间，平均链锁聚类分析可将26种鹅膏菌分为二大类，且一些具菌环和苞状菌托的种类聚在一起，与形态分类基本相吻合。
Spearman analysis showed that the improvement of bone density after treatment was closely associated with serum OPG level.
Spearman相关分析显示治疗前、后骨密度的改变与OPG的变化有关。
This result revealed that the mutation of OPG isoform was not of interorgan difference.
说明这一变体基因并非是组织间差异。
Both OPG and RANKL are important for the regulation of osteoclastic function.
护骨素/核转录因子κB受体活化素配体在破骨功能调控中起重要作用。
Objective: To investigate the expression of OPG and OPGL in osteosarcoma and its significance.
目的：探讨OPG及OPGL在骨肉瘤中的表达及意义。
But OPG expression seemed not to be regulated.
其OPG的表达没有显著差异。
After induced by rhBMP, the expression of IGF-1 and OPG has no significant difference between two groups.
4：rhBMP-2诱导后，两组骨髓基质干细胞IGF、OPG的表达量没有显著差别。
The functional OPG protein was expressed in eukaryotic cells, but it is difficult to obtain sufficient amount of protein.
真核细胞能够表达具有生物功能的OPG，但存在的普遍问题是表达量不高。
Installed with modern OPG X-Ray machines, we are able to make diagnoses accurately and provide comprehensive treatments for you.
我们也安装了自己的全景X-光机设备，可为您提供快速正确的诊断及全方位的治疗服务。
The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods, the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.
1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。
And it also regulates root resorption by expression of OPG and RANKL, which influence osteoclastogenesis directly.
关于正畸力作用下成牙骨质细胞的功能受到了怎样的影响，以及其与OIIRR的发生发展有什么样的联系，在国内外少见报道。
The expression of OPG may have a close correlation with the ossifying of osteosarcoma.
骨肉瘤中存在OPG高表达，可能与骨肉瘤的成骨性有关。
Given the age of onset and limitation of cherubism to the maxilla and mandible, it is possible that SH3BP2 is involved with regulating the skeletal remodeling that accompanies proper tooth development. The present study includes the following parts: Part Ⅰ 1.50 cases of central giant cell lesion of the jaws and 50 cases of giant cell tumor of long bones were used for clinicopathologic study.
检测了RANKL蛋白和OPG蛋白在颌骨GCL和长骨GCT中的表达，结果表明RANKL在血管内皮细胞强烈表达，提示激活的血管内皮细胞有助于通过调节RANKL的表达来促进多核破骨样巨细胞的形成；另外，在纤维基质，骨基质和个别多核巨细胞的胞膜也有RANKL的表达，提示可溶性的RANKL以旁分泌、自分泌和内分泌的方式发挥作用。
Tooth eruption; Osteoprotegerin; Tooth germ; Dental sac
牙齿萌出；骨保护素OPG ；牙胚；牙囊
Their expression of the above-mentioned genes is very important in the process of osteogenesis, bone growth, bone fracture healing and bone remodeling and are markers of the natural bone-formation.
成骨细胞骨重建相关的RANKL/OPG系统对于成骨分化过程中的骨重建具有十分重要的意义。
OVX model of S-D rats was established. Bone mineral density and the levels of mRNA and protein of RANKL and OPG in condyles of femur were detected at different times. The relationship between the expression of RANKL and OPG and osteoporosis was investigated.
3建立去卵巢大鼠模型，于术后不同时间点取股骨髁，检测骨组织RANKL、OPG的mRNA、蛋白表达情况，测量股骨髁的骨密度，观察RANKL、OPG表达与骨质疏松的关系。
Immunocytochemistry was used to test the expression of RANKL in hPDLCs at protein level.
研究二 人牙周膜细胞在间歇性牵张力作用下OPG的表达。
However, it is not clear whether OPG and RANKL participate in the progress of HPDLCs'differentiation into osteoblasts.
在人牙周膜细胞向成骨细胞分化的过程中是否有OPG和RANKL的参与，目前尚未见报道。
Levels of RANKL and OPG mRNA were detected with real-time quantitative RT-PCR.
结果：高剂量和生理剂量E2均下调hPDLCs的RANKL mRNA的表达水平。
OPG belongs to the TNF superfamily and its ligand, RANKL, is a member of TNF ligand family.
OPG的配体是一个已知的TNF配体家族的成员RANKL。