DLA-EIAV can be served as the animal model for studying on human immunodeficiency virus. The vaccine was developed in two stages. A carefully selected field isolate, EIAV LN, was passaged 133 times in vivo in donkeys to generate the highly virulent and pathogenic strain D510, which kills animals within a few weeks. D510 went through 135 passages in vitro to obtain vaccine strains DLV. In order to investigate the molecular mechanism of the attenuation and protection of donkey leukocyte attenuated equine infectious anemia virus, we investigated the evolution of LTR of 24 out of 110 passages during Chinese EIAV attenuation.
马传贫弱毒疫苗致弱路线是：将一株来源于马体的EIAV 超强毒力的辽毒株经过驴体连续传代100 余代使病毒毒力增强后获得了驴强毒株(对马和驴均100%致死)，然后将DV 株连续通过驴白细胞进行体外培养驯化，使其病毒毒力完全丧失，而保持了良好的免疫原性，最终培育成功EIAV 驴白细胞弱毒疫苗(沈荣显等，1979)，DLA进一步通过驴胎皮肤细胞培养驯化，又获得了皮肤细胞弱毒疫苗株。
The total length of CFG1 element is 4826 bp, including 5`-LTR (192 bp), entire ORF (4047 bp) and 3`-LTR (189 bp).
CFG1元件的全长为4826bp，包括5`-LTR(192bp)，完整的ORF(4047bp)和3`-LTR(189bp)。
This paper presents the method of LQG/LTR multivariable controller design of a gust load alleviation system.
给出了阵风载荷减缓系统LQG/LTR多变量控制器设计方法，以某大型运输机为对象，设计了阵风载荷减缓系统控制器。
My houes:dun wan to tell u ltr come suri barang saya.
我家：不想根你讲，怕你来偷东西
It achieves 90-97% accuracy in the classification of novel DNA and LTR repeats, and 75% for LINEs and SINEs.
在对新的DNA和LTR重复进行分类时，获得了90-97%的准确率，对LINEs和SINEs进行分类时，获得了75%的准确率。
They were sequenced and compared with other isolates from different areas of the world and constructed the phylogenetic tree.
用PCR的方法扩增福建 5株样品的Env及LTR区基因，测序后与来自世界各地的代表株进行比较、分析，构建进化树。
LTR of DLA-EIAV was cloned into upstream of bacterial CAT gene. The resultingrecombinant plasmid was designated as pCAT-LTR. Standard trans-activition assaysutilizing the EIAV LTR-driven CAT gene were performed in fetal donkey dermalcells, TJ-905 cells, donkey leucocytes, EIAV-infected FDD cells and EIAV-infectedDL, respectively. CAT gene under the control of LTR was expressed at different levels inthese cells. The results showed that the LTR of DLA-EIAV could promote gene expressionin FDD, TJ-905 and DL cells, and can be trans-activeted in FDD and DL cells.
将我国马传贫病毒弱毒株的长末端重复序列克隆于含CAT基因的载体中，获得重组质粒pCAT-LTR，用pCAT-LTR分别转染驴胎皮肤细胞、胶质母细胞瘤(TJ-905)细胞、驴白细胞及接种EIAV弱毒的FDD和驴白细胞，结果以EIAV弱毒的LTR为启动子，CAT在这几种细胞中均获得了不同程度的表达，证实了我国EIAV弱毒株的LTR在FDD、TJ-905及驴白细胞中确实具有一定的启动子功能，并能被反式激活表达。
So are you serious about a ltr/marriage?
所以你严重的约1升/婚姻？
The consequences of such widespread LTR activation are currently still unclear, according to the study's authors.
据该研究的作者介绍，这种广泛的LTR激活的后果目前尚不明朗。
Further more, one can find that the H_/LTR method h...
推进了多变量鲁棒控制设计方法在航空发动机中的实际应用。
Iam a positive romantic man who is looking for someone who is interested in a LTR leading to marraige.
我是个积极浪漫的男人，寻找一个想结婚的女人。
It was concluded that LTR was an contribution to the cell tropism and virulence of EIAV.
实验表明LTR是EIAV细胞嗜性及毒力的影响因素之一。
Truth is, no one in a successful LTR knew for sure that it was true and lasting love until it lasted.
事实是，即使是成功的长期关系里的人们不到最后一刻也不能肯定地说这真爱将持续到永久。
Simulation results of LTR control for a turbofan engine show the effectiveness of the approach.
将此设计方法应用于某型涡扇发动机的LTR控制，取得了良好的控制效果。
It is a big Ty3-like retrotransposon with long LTR sequence.
RIRE10是一个有长LTR区的大型Ty3类逆转录转座子。
Sativa, has undergone recent bursts of three LTR-retrotransposon families. This genome has accumulated more than 90, 000 retrotransposon copies during the last three million years, leading to a rapid twofold increase of its size.
然而，Piegu等人的研究却发现，该基因组在过去的3百万年间，积累了大约90000个逆转录转座子，这使得澳洲野生稻的基因组大小几乎增加了一倍。
JDV Tat can transact BIV, BFV and BLV LTR. So the interaction of different viruses may be the important reason of diseases caused by virus.
JDV的Tat可以反式激活BIV、BFV、BLV基因的表达，病毒的相互作用可能是慢病毒致病的重要素。
Rous Sarcoma Virus enhancer/promoter, modified HIV-15'and 3'Long Terminal Repeat, HIV-1 psi packageing sequence, HIV Rev response element were amplified by PCR, then partial goat β-casein promoter, partial goat β-casein genomic sequence and pro-UK(subscript M13) cDNA were used to construct lentivirus expression vector specific for mammary gland. And its expression specificity was proved by transfection the MCF-7 and CHO cell in vitro and directly injecting the vector into the lactating mammary glands of goats.
方法］将劳氏肉瘤病毒增强子／启动子、复制缺陷型艾滋病病毒(HIV-1)的5'端长重复序列、HIV-1Ψ包装信号、HIV Rev反应元件、山羊β-casein调控序列、Pro-UK(下标 M13)、△U3/3' LTR依次连接，构建乳腺特异性表达的慢病毒载体；通过体外转染人乳腺癌细胞系(MCF-7)、仓鼠卵巢细胞和泌乳山羊乳腺注射证明其表达有效性。
The application of modified nylon 66 cord in LTR tire was investigated and compared to that of HMLS polyester cord.
研究改性锦纶66帘布在半钢子午线轮胎中的应用，并与高模量低收缩聚酯帘布进行比较。
A recombinant pl asmid (designated as p8.0) was obtaining by ligating these three fragments. The p8.0 was subcloned into a plasmid (designated as p8.2) containing entire LTR of EIAV DLA. The complete nucleotide sequence of DLA strain of EIAV was determined by sequencing the p 8.2. The purified p8.2 was used to transfect donkey leuko cyte cultures.
将此8.0kb EIAV全基因再亚克隆到含有一完整EIAV DLA株长末端重复序列的质粒中，获得一含有EIAV驴白细胞弱毒前病毒全基因的重组质粒，将其命名为p8.2，经核苷酸序列分析，证明p8.2含有EIAV前病毒的全基因。