Objective The aim of this study is to investigate the function of Golgi apparatus during in vitro development of mouse oocytes.
目的初步探讨高尔基体在小鼠卵母细胞体外发育进程中的作用。
In contrast, the Golgi apparatus of most animal cells is a continuous network of membranes.
相比之下大多数动物的高尔基器是连续的膜的网状结构。
Membrane remodelling1, 2, 3, 4, 5 plays an important role in cellular tasks such as endocytosis, vesiculation and protein sorting, and in the biogenesis of organelles such as the endoplasmic reticulum or the Golgi apparatus.
生物谷报道：细胞膜远不止是细胞外的一层包裹物，对其拓扑进行的重塑研究，将它们与内吞作用、囊泡形成和蛋白分选等重要功能联系了起来。
Prokaryotes lack organelles such as nucleoli, mitochondria, plastid s, Golgi apparatus, and do not exhibit cyclosis.
原核生物无细胞器，如核仁、线粒体、质体、高尔基体等，不出现胞质环流。
AAR19081. We show that this molecule was unbiquitously expressed in all the tissues examined and located mainly in Golgi apparatus as shown by RT-PCR and subcellular localization experiments. In addition, we found that over-expression of this molecule in NIH-3T3 cells induced an inclusion cells phenotype characterized by intra-cytosol vacuoles as shown by phase-contrast microscopy observation, and dramatic increase of lysosomes as shown by electron microscope. The activity of lysosomal enzymes (β-Glucuronidase and β-galactosidase) decreased in cytosol and increased in the cultural media, as detected by 4-MUG (4-methylumbelliferyl-D-glucuronide) and ONPG (o-nitrophenyl galactopyranoside) regents, respectively. These results suggested that HYP36 might be involved in the sorting process of lysosomal enzymes.
，该基因广泛表达小鼠各种组织、细胞；亚细胞定位分析显示，该分子主要定位于高尔基器；转染NIH-3T3细胞可以诱导细胞产生I-细胞表型：相差显微镜观察可见细胞中有很多胞质小泡，亚细胞结构观察显示细胞内出现大量脂滴，溶酶体大量增生，生化指标检测显示细胞内溶酶体酶（β-葡萄糖醛酸苷酶和β-半乳糖苷酶）的含量降低，细胞培养上清中溶酶体酶的含量上升，提示HYP36可能参与了细胞内溶酶体酶的分选过程。
The intracellular site of origin for sebum has never been determined. It has been assumed that they originates from the endoplasmic reticulum or from the Golgi apparatus, or even mitochondria, but there has never been a convincing demonstration of either site by electron microscopy or by any other techniques. In the present work, we investigate the synthesis of lipid droplets by addition of the PPAR? _ activator to the cultured sebocytes. By Nile red stain, immunofluoresence examination of endoplasmic reticulum and Golgi apparatus, and ruthenium tetroxide examination, the synthesis of lipid droplets was located within the smooth endoplasmic reticulum.
而脂腺细胞在这一方面之研究并不清楚，早期之报告认为内质网状体或高尔基氏体，甚至粒腺体为皮脂产生之处；我们利用最近建立之培养皮脂细胞分化模式为基础，以 Linoleic acid 活化 PPARδ转录因子，诱导培养人类皮脂细胞之脂肪合成，在 Nile red 之中性脂肪染色及内质网状体之免疫萤光染色下可观察到脂肪滴之分布与内质网状体有一致性之关系，而与高尔基氏体之分布无观，进一步以 Ruthenium tetroxide 染色，在电子显微镜下观察上述细胞脂肪之合成部位显示滑面内质网状体为人类脂腺细胞脂肪滴之合成部位。
Hyalinocytes can phagocytize the granulocytes which have expelled their granules. In hyalinocytes, ACP was found synthesized in Golgi apparatus and was involved in the endocellular digestion process.
透明细胞可以吞噬排放颗粒后的颗粒细胞，透明细胞中由高尔基体合成的酸性磷酸酶等溶酶体酶主要用于透明细胞的细胞内消化作用。
In the case of the artificial Golgi apparatus, this mean that a molecule can be exposed to a series of enzymes or other relevant molecules, one at a time—and that the order of exposure can be changed at the whim of the experimenter.
就人工高尔基体来说，这就意味着一个分子可以同时接触到一系列的酶或其他相关分子，而接触顺序则全凭实验者的个人意愿。
It consists of stacks of flattened membrane-bounded sacs that bud off vesicles containing the products of the secretory activity in the Golgi apparatus.
在成熟的囊中含有高尔基体活动时产生的分泌物。
The uptake inhibition experiment results showed that Lf-NP_ wastransport into brain by an energy-dependent process involving LfR, caveolae, clathrin, golgi apparatus, lysosome and macropinocytosis.
摄取抑制实验的结果证实，Lf-NP_是由LfR转运入脑，通过穴样凹陷，包被凹陷和巨胞饮途径内吞，为高尔基体和溶酶体参与的能量依赖过程。
KLEIP is present in both cell-cell contact and Golgi apparatus.
在本篇论文中，我们找出了KLEIP在细胞中的所在位置。
The changes of Golgi apparatus during spermatogenesis of Macrobrachium nipponense were studied with transmission electron microscopy.
用透射电镜技术研究了日本沼虾精子发生过程中生精细胞内高尔基体的变化。
The present studies showed that two cell populations were found in haemocytes: large cell with high granularity and small cell with low granularity by flow cytometry FCM on light scanttering pattern. Two distinct cell types were identified based on phase contrast microscope: one type of cell was dark and dioptric aberration, while the other was bright and dioptric strong. By Giemsa and H. E staining, cytoplasmic staining were heterogeneous and internal particles were obvious in one type of cell, while cytoplastic staining were homogeneous and internal particles were inexistent in the other type of cell. By transmission electron microscope, we found that the mitochondria, Golgi apparatus organelles were rich and internal particles were obvious in one type of cells, and contrary to the another cells.
流式细胞术光散射图谱显示血细胞被分两类，一类为颗粒度高的大细胞，另外一类为颗粒度低的小细胞；相差显微镜观察显示，血细胞可分为胞体暗、折光性差和胞体明亮、折光性强的两类； Giemsa和H.E染色显示细胞分为胞质染色不均一、胞内颗粒明显和胞质染色均一、胞内颗粒不明显的两类；透射电镜超薄切片观察显示，颗粒明显的细胞胞质内线粒体、高尔基体等细胞器较丰富，颗粒不明显的细胞胞质内细胞器较少；负染结果表明血细胞主要分为表面不光滑、突起明显和细胞表面光滑、突起较不明显的两类。
Recently, our laboratory has found that PRMT5 localizes to and affects Golgi Apparatus.
近来，我们实验室发现PRMT5定位于高尔基体并影响其结构。
Mitochondria was relatively little in size. Round primary lysosome with high electron-densed granules and secondary lysosome with high or low electron-densed granules were seen frequently. DCs contained many rough endoplasmic reticulum, the Golgi apparatus and ribosomes. The vacuoles with flocculent electron-densed granules were rare. Some special granules in cytoplasm were seen, whose surface like earphone were covered with a membrane. High electron-densed contents in the granules were near one side and the other side was bright. The nucleus became markedly small in volume, nephroid or hoofed in shape. The nucleus had little euchromatin and lots of heterochromatin under nuclear membrane.
子宫内膜癌组织DC超微结构特征如下：细胞形态不规则，与正常子宫内膜组织DC相比，胞膜较光滑，胞膜表面树突状胞浆突起显著减少，部分突起呈粗短状；胞质中线粒体相对少，圆形而电子密度高的初级溶酶体和不规则形且电子密度高低不一的次级溶酶体多见；高尔基体、粗面内质网、核糖体丰富；含微量絮状电子致密物的胞饮小泡显著减少；胞质中可见形态特殊的颗粒，该颗粒外周膜包裹，略呈圆形，中间部位稍弯曲，如耳机状，颗粒中由高电子致密物居于一侧，而另一侧则呈透亮状；胞核显著减小，居于胞质一侧，常呈肾形或马蹄形，核内常染色质较少，异染色质多边集于核膜下。
The ultrastructure showed numerous digital processes, moderate amount of rough and smooth endoplasmic reticulum, rich Golgi s apparatus, microfil-aments, few lysosomes without myofilaments and dense bodies.
超微结构显示，细胞表面有许多指状突起，细胞含有中等量滑面型、粗面型内质网，高尔基器丰富，胞浆内溶体含量少，有微丝，无肌丝和致密斑。
The proteins they make can enter the cisternae for transport to other parts of the cell or for secretion via the GOLGI APPARATUS.
此处产生的蛋白质可以直接进入扁平囊进行修饰而后输送到细胞的其他部位，或送到高尔基体进一步加工再被分泌。
Cisternae A flattened membrane-bounded sac of the endoplasmic reticulum or the Golgi apparatus, being the basic structural unit of these organelles.
潴泡：在内质网或高尔基体中存在的扁平的膜状囊泡，是这些细胞器的基本结构单位。
The Golgi apparatus is also the site where synthesis of polysaccharides, e. g.
高尔基体还是进行多糖类合成的场所，如粘液中的多糖的合成。
The point of all this is that, although it is known which enzymes are active in a natural Golgi apparatus, the details of how the modifications happen are obscure.
这前面所有一切的关键是，尽管人们知道酶在天然高尔基体中起一定的作用，但具体怎样发生作用却仍是未知的。