The fusion protein GST-hEGF expressed in Escherichia coli in the experiments with mice showed the recombinant protein had good immunogenecity and the purifid protein hEGF could stimulate the growth of Hela cells efficiently.
用原核表达得到的融合蛋白进行了小鼠试验，证实其具有很高的免疫原性；用纯化得到的hEGF进行细胞活性检测，表明其能促进细胞生长。
The constuction of plant expression vector, tissue culture of cabbage and gene transformation conditions were studied to obtain ubiC transformants. ubiC gene was obtained from the Escherichia coli. by PCR amplication and was ligated to pUC118 vector.
为获得转ubiC基因甘蓝，对植物基因工程载体的构建、甘蓝的组织培养以及基因转化条件进行了研究。
Brinkmann U%Mattes RE%Buckel P High-level Expression of Recombinant Genes in Escherichia coli is Dependent on the Availability of the dnaY Gene Product 10.1016/0378-1119(89)90470-8 Gene, 1989, 1
隋广超%刘芳%胡美浩利用噬菌体T7RNA聚合酶在大肠杆菌中引导尿激酶原克隆基因的表达北京大学学报，1994，6
After bleeding sloped, the hemoglobin amount in films or gauzes were measured to determine the hemostatic effect Using a randomized design, one incision in each animal was treated with C-CM membrane, the influence of C-CM film on the growth of bacteria (Staphylococcus aureus ATCC 27853. Escherichia coli ATCC 25922. Monil lia albicans ATCC 14053)were observed in vitro with solid medium. Rusults: It was observed that C-CM membrane had no toxic effects on surrounding tissue and was slowly degraded after implanted into rat leg muscular porch; The results of hemostatic experiment showed that C-CM film adhered well to incisions.
结果：C-CM膜植入动物肌肉中，周围组织无明显炎症，未发现组织变性和坏死，可在组织内缓慢降解，具有良好的组织相容性，6周后反应接近外科缝合丝线组；止血实验结果发现：C-CM膜组和纱布组的出血时间分别为83.5±9.333s和117±15.9275s，血红蛋白光度吸收值为0.9204±0.7559和1.9764±0.7205，二组比较差异显著(P＜0.05)，说明C-CM膜有一定的止血作用；促进创面愈合实验结果显示：不同时间段C-CM膜组肉芽组织较对照组厚，其中胶原纤维含量亦较多，C-CM膜组的创面愈合时间早于空白对照组，伤口愈合较快；抑菌实验显示C-CM膜对三种菌产生接触性抑制。
Epidermidis、Sarcina lutea、Bacillus subtilis and Escherichia coli with MIC of 31.3 μg/mL, 31.3 μg/mL, 7.8 μg/mL, 7.8 μg/mL and 125.0 μg/mL, respectively. It can also suppress the growth of Candida albicans with MIC of 125.0 μg/mL.
epidermidis、Sarcina lutea、Bacillus subtilis和Escherichia coli的MIC分别为31.3 μg/mL、31.3 μg/mL、7.8 μg/mL、7.8 μg/mL和125.0 μg/mL；对真菌Candida albicans的MIC为125.0 μg/mL。
The result expressed that under the concentration of 0.2mg/mL, the 6 compounds are all active to Sarcina lutea, ME-1, MT-6, MT-land MT-7 are active to Bacillus megaterium, to Escherichia coli, only MT-2 is active. The dimeric natural products are intensively existed in botany, most of them have the obvious biological activities. They have been concentrated on in recent years, and they are one of the compounds to which are enjoyed great popularity in natural product.
结果表明，在浓度为0.2mg/mL的条件下，六种化合物对藤黄八叠球菌均具有明显抑制作用，ME-1、MT-6、MT-1、MT-7对巨大芽孢杆菌有抑制作用，MT-2对大肠杆菌有抑制作用，对绿脓杆菌和枯草芽孢杆菌六种成分均无抑制作用；二聚体化合物在植物界广泛存在，多数二聚体天然产物具有显著的生物活性，引起人们的重视，是天然产物化学界研究的热门化合物之一。
Bactericidal efficiency of vinegar against Staphylococcus, Escherichia, Candida albicans and natural bacteria was extremely intensive with stable property.
食醋对金黄色葡萄球菌、大肠杆菌、白色念珠菌和自然菌有极强的杀灭效果，且性能稳定。
To explore the effect of Senecio Scandens Buch-Ham on the biosynthesis of Staphylococcus aureus and Escherichia coil by serum pharmacological method.
目的 应用血清药理学方法研究中药千里光对金黄色葡萄球菌和大肠埃希菌生物合成的影响。
All have antibacterial activities(MIC 2.50 mg/mL). The antibacterial activity of acetic acid ester extg. to Staphylococcus aureus and Escherichia coli was evident (MIC=0.625 mg/mL), the antibacterial activities of ethanol extg. and n- butylol extg. to Staphylococcus aureus were better(MIC=2.50mg/mL).To study the relieving alcohol toxicity effect and liver protection effect of Ilexlatifolia Thunb.
结果表明枸骨叶中的3种溶剂组分(乙醇提取物、正丁醇和乙酸乙酯萃取物)均显示一定的抑菌效用(MIC≤2.50mg／mL)，其乙酸乙酯萃取物对金黄色葡萄球菌和大肠埃希氏菌的抑菌效果最为显著MIC为0.625mg／mL，醇提物和正丁醇萃取物对金黄色葡萄球菌也显示较好的抑菌效果MIC均为2.50 mg／mL。
Various genomic DNAs of Listeria innocua, Listeria weshimeri, Salmonella, Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Proteus were used as negative controls to confirm the specificity of duplex real-time PCR assay.
结果 成功构建了重组质粒标准品，并运用5'、3'端分别标记FAM、TAMRA的hly基因探针和5'、3'端分别标记HEX、TAMRA的ipaH基因探针成功建立了单增李斯特菌与志贺菌同步荧光定量PCR检测方法。
Many recombinant hydroxynitrile lyases have been expressed in Escherichia coli, Saccharoinyces cerevisiae and Pichia pastoris.
许多重组醇腈酶业已在大肠杆菌，啤酒酵母和甲醇酵母中成功表达。
Staphylococcus aureus, beta streptococcus, streptococcus pneumoniae, Branhamella catarrhlis and Escherichia coli have been isolated in the NGU group but not in the controls.
但NGU组还分离出对照组所没有的金黄色葡萄球菌、乙型链球菌、肺炎链球菌、卡他布兰汉菌和大肠杆菌
In this study, we efficiently expressed the active antimicrobial peptide, which fused with the site-mutated coat protein of the classical swine fever virus, in Escherichia coli.
本研究采用猪瘟病毒(Classical swine fever virus，CSFV)定点突变外壳蛋白为融合蛋白，对抗菌肽Cecropin AD基因进行了高效融合表达，获得了有抗菌活性的抗菌肽CAD。
Objective To investigate the change of outer membrane permeability in multiple-antibiotic-resistant strains of Escherichia coli.
朱德妹 ，汪复，张小林目的研究临床分离的多重耐药大肠杆菌耐药的外膜屏障机制。
Objective To investigate the active efflux system of antibiotics in multiple-antibiotic-resistant Escherichia coli.
朱德妹 ，汪复，张小林目的研究主动外排系统在多重耐药大肠杆菌中的作用。
Results All mice of control group died within 24 hours after the injection of bacteria, while those of the therapy group nere part survived. The ED50 and 95％confidence interval of Danshitongjiaonang against Staphylococcus and Escherichia coli was 215 mg/kg, 143～316 mg/kg and 1309 mg/kg, 771～4591mg/kg.
结果 对照组小白鼠在24 h内全部死亡，治疗组则可部分存活，胆石通胶囊对金黄色葡萄球菌和大肠杆菌的ED50和95%可信限分别为215 mg/kg、143～316 mg/kg和1309 mg/kg、771～4591 mg/kg。
While stimulated by Pasturella multocida and Escherichia. coli O_78, the content of NSE changed not so dramatically.
而受巴氏杆菌、沙门氏菌刺激后引起的变化不明显。
After stimulated by Escherichia. coli O_78 and Pasturella multocida, the contents of CD54 decreased greatly in part of SR.
大肠杆菌和沙门氏菌只在局部引起CD54表达量急剧降低。
After stimulated by Staphylococcus aureus, Escherichia xoli O_78, Pasturella multocida, the content of gastrin in follicle increased significantly.
结果如下：1。离体圆小囊受葡萄球菌、大肠杆菌、巴氏杆菌细菌抗原刺激后，胃泌素在圆小囊淋巴滤泡部位的表达，有显著升高的变化。
MethodsThe agar diffusion and tube dilution methods were used to study the growth on bacteria inhibition of the Lonicerae extracts with five different kinds of extraction approaches, which were SFE extraction, SFE-C extraction, alcohol extraction, ultrasonic extraction and SD extraction, by applying them to the following bacteria: Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Staphylococcus albus, Pseudomonas aeruginosa, Salmonella typhimurium.
方法采用琼脂扩散法和试管稀释法研究金银花的5种提取工艺，即SFE萃取、SFE-C萃取、乙醇提取、超声波提取、共水蒸馏法提取，所得提取原液药物对6种细菌，即金黄色葡萄球菌、大肠杆菌、枯*芽孢杆菌、白色葡萄球菌、绿脓杆菌、鼠伤寒沙门氏菌的抑菌作用。