The results are as follows:(1) the amplituds of the tail currents increased from 9.3 ±2.8 pA to 64.1 ± 3.4 pA (P.001) when the depolarizing duration was increased from 1 ms to 180 ms; (2) the amplitudes 0f the tail current were reduced when repolarizing pulses were applied, with a reversal potential of about-63 mV; (3) the amplitude of the tail current was significantly depressed or even almost completely blocked by extracellularly applied 500 #mu#mol/L Cd~(2+) or intracellularly applied 11 mmol/L EGTA; (4) the amplitudes of the tail currents were decreased by about (26.32±3.9)%(P.01) after 200 nmmol/L apamin was extracellularly applied; and (5) the fast component of the tail current was sensitive to 10 mmol/L TEA while the slow component was insensitive.
结果发现：随着去极化时间从1ms延长至180ms时，尾电流幅度由9.3±2.8pA逐渐增大至64.1±3.4pA(P＜0.001)；当去极化结束后的复极化电位降低时，尾电流幅度先逐渐下降到零，然后改变方向，逆转电位约为-63mV；细胞外施加500μmol／LCd~(2+)或细胞内液中施加11mmol／LEGTA时尾电流明显减小甚至完全消失；尾电流中慢成分的幅度在细胞外给与200nmol／L蜂毒明肽后，减小了约26.32±3.9％(P＜0.01)；细胞外施加10mmol／L TEA，可明显降低尾电流中的快成分。结果提示，在DRG神经元启超极化中存在Ca~(2+)激活K~电流的蜂毒明肽敏感成分──I_。
Therefore, we forced cells to suspend or included EGTA in suspended cell, and examine their sensitity to anoikis.
所以，我们接著以迫使细胞悬浮，或者再另外加入EGTA减少细胞之间的黏附，以检测细胞对此种处理所诱发凋亡之敏感性。
The presence of EGTA obviously enhanced the inhibition of TFP on the viability of the decidual cells.
EGTA可增强三氟拉嗪对细胞活力的抑制作用。
There was a positive tendency between the increase times of POX, CAT and APXactivities induced by salt treatment and the relative growth rate of all cell lines, but a negative one ofSOD.
AH33细胞在盐处理和Ca2+处理下POX活性持续增加，EGTA和CoCl2也抑制这一刺激效应，但TFP不再有促进效应，表明Ca2+可能也是该细胞POX活性增加所必需，且不受活化CaM抑制。
Thapsigargin has stronger inhibition effect than EGTA in two proteins and more early.
Thapsigargin比起EGTA对於两种蛋白质的基因表现有比较明显的抑制，且时间点也比较早。
However, EGTA, nicardipine, thapsigargin, ryanodine and herbimycin A had no effect.
LPA浓度依赖地促进VSMC增殖，其细胞内信号转导与PTX敏感的G蛋白介导的蛋白激酶C和MAPK途径有关。
RESULTS:(1)0.06 μmol egtazic acid intracerebroventricularly infused induced a rise in colonic temperature, when PMZ(5 mg/kg) was administrated intravenously 20 min after the infusion of egtazic acid, it reduced the febrile responses of rabbits to egtazic acid significantly (0.06 μmol) but without complete suppression.
结果：(1)侧脑室灌注0.6 μmol EGTA引起家兔明显的发热反应，侧脑室灌注0.6 μmol EGTA 20 min 后，静脉注射PMZ(5 mg/kg)明显抑制EGTA引起的结肠温度上升，其3 h发热反应指数明显低于侧脑室灌注0.6 μmol EGTA 20 min后静脉注射生理盐水组。
PMZ possesses antipyretic effect on egtazic acid-induced fever in the rabbit and raises the possibility that the antipyretic actions of PMZ are mediated through the increase of ［Ca2＋］i incuced by PMZ.
PMZ诱导体温调节中枢神经细胞［Ca2＋］i升高可能是PMZ抑制家兔EGTA性发热的中枢机制之一。
5MM EGTA and 58mM K〓were found to affect the GH secretion but not the GH mRNA level stimulated by GHRF, showed that GHRF stimulate GH gene expression independently of GH release.
但是，Forskolin对GHRF所诱导的和基础的鲤鱼脑垂体GH分泌和GH mRNA水平均有刺激作用，表明cAMP在鲤鱼GH分泌和基因表达的调控中起着重要的作用。
In the present study, we found that EDTA. EGTA and GST-calpastatin inhibited myoblast fusion.
本研究发现EDTA、EGTA、GST-calpastatin可抑制成肌细胞的融合。
In Ca2+-free Krebs solution containing 0.01 mmol·L-1 EGTA, resveratrol and phloretin inhibited NE-induced contraction which was caused by Ca2+ release from intracellular store, but did not affect the contraction which was induced by Ca2+ influx.
在无钙Krebs液(含0.01 mmol·L-1 EGTA)中，白藜芦醇和根皮素可抑制NE诱发的由肌细胞内钙释放引起的Ⅰ相收缩，但不影响CaCl2诱发的由肌细胞外钙内流引起的Ⅱ相收缩。
Experimentally, stores can be emptied by increasing of InsP3 molecule in the cytosol, dialyzing the cytoplasm to high concentrations of Ca(superscript 2+) chelators such as EGTA or BAPTA, or treating with the endoplasmic/sarcoplasmic reticulum Ca(superscript 2+) ATPase inhibitors like thapsigargin and cyclopiazonic acid.
尽管这个领域受到愈来愈多的重视，然而，钙池调控钙离子通道所能影响的细胞生理意义或其调控机制，至今所知仍然非常有限。
Original current traces showed that the inactivation process was slowed in the presence of ryanodine or high concentration of Ca〓 chelator EGTA.
原始的电流曲线提示，在Rya或电极液中高EGTA存在的条件下，Str 1μmol/L使I〓的失活过程减慢。
A practical method including following steps for the isolation and primary culture of rat pancreatic β cells has been developed: isolating islets from rat pancreas with collagenase type V; incubating islets with EGTA; digesting islets with low concentration of trypsin and DNase I; and then centrifugalizing, washing, collecting and culturing single cells.
以V型胶原酶分离胰腺得到胰岛，在EGTA预孵育后以低浓度的胰蛋白酶和DNaseI酶联用消化胰岛，建立了大鼠胰腺β细胞的分离及原代培养方法。反复实验表明（n＝10），所获得的细胞表面光洁、完整、呈球形，功能正常，能对生理剌激物迅速反应，适用于通常的单个细胞的电生理学实验
In these three targets, the EGTA processing displays the intense inhibitory action, it means that the lacks of the calcium ion to produce more injury in pepper`s seedlings.
三个指标中外加EGTA的处理均表现出强烈的抑制作用，说明它螯合钙离子后产生的缺钙伤害较大。
Antimonate calcium precipitates in the cell wall of hyphae grown for 5h in pH 6.8 medium containing 2mmol/L EGTA was reduced a little.
在含有2mmol/L EGTA的pH6.8介质中培养5h后的菌丝，最顶端细胞壁焦锑酸钙沉淀稍有减少。
2During chilling stress, Ca~(2+)treatment could delay the decrease of the activity of SOD, POD and CAT; And it could delay the decrease of the content of GSH, AsA and Car of the heat-shock-induced cucmber seedings. Therefore it enhanced the antioxydization ability, and reduced malon-diadehyde(product of membrane lipid peroxidation)and cytomembrane permeability of the heat-shock-induced cucmber seedings.
2钙处理能减缓冷胁迫下热激黄瓜幼苗的SOD、POD和CAT的活性以及GSH、AsA、Car的含量下降，因而增强了热激黄瓜幼苗的抗氧化能力，降低了膜脂过氧化产物丙二醛含量和质膜透性；而EGTA处理则相反。
But the EGTA treatment had the opposite effect.(3)During chilling stress, Ca~(2+)treatment could delay the decrease of K~+, Mg~(2+), soluable protein content and the increase of Ca~(2+)content; And it could enhance the increase of soluable sugar, free amino acid, proline, heat stable protein and Na~+ content of the heat-shock-induced cucmber seedings. Then it enhanced the osmoregulation ability and delayed the decrease of water potential of the heat-shock-induced cucmber seedings. But the EGTA treatment had the opposite effect.
3钙处理能减缓冷胁迫下热激黄瓜幼苗的可溶性蛋白质、K~+、Mg~(2+)含量的下降以及Ca~(2+)含量的上升，增强可溶性糖、游离氨基酸、脯氨酸、热稳定蛋白和Na~+含量的上升，增强了热激黄瓜幼苗的渗透调节能力，从而缓解了低温下水势的下降；而EGTA处理则相反。
4During chilling stress, Ca~(2+)treatment could delay the decrease of chlorophyll content and RuBPCase activity; And it could enhance Pn, Gs, Tr, the saturation point and dark respiratory speed of the heat-shock-induced cucmber seedings. So it reduced Ci. But the EGTA treatment had the opposite effect.
4钙处理能减缓热激黄瓜幼苗冷胁迫下叶绿素含量和RuBPCase活性下降，提高Pn、Gs、Tr、光补偿点和暗呼吸速率，降低Ci；而EGTA处理则相反。
The antibody against S. pombe CaM, TFP and Phenyl-Sepharose CL-4B could decrease the inhibition of CaM on the S. pombe proliferation. While Ca2 + and EGTA had no effects.
抗粟酒裂殖酵母CaM抗体、TFP及Phenyl-SepharoseCL－4B能降低CaM对细胞生长的抑制作用，而Ca2+及Ca2+螫合剂EGTA对CaM的抑制作用均无影响。