17 cases well-differentiated/dedifferentiated liposarcoma MDM2 and CDK4 express difference unusually is 76% and 82%.
1.17例高分化／去分化脂肪肉瘤中MDM2和CDK4异常免疫表达分别为76％和82％。
These diabetes mice remain diabetic for more than 2 weeks after injection of STZ. During this time, we did not observe any spontaneously reversion of hyperglycemia in these diabetic mice. Moreover, we investigated the changes of islet size and expression of several proteins related to β cell proliferation and apoptosis after treatment with 250mg/kg STZ from 0 to 4 hours.
研究结果表明：在高剂量的STZ（250mg/kg）注射后，小鼠以高比率发生糖尿病，而且可以在2周以上的时间内稳定地维持糖尿病症状，其间并没有观察到血糖的自发恢复；在注射STZ（250mg/kg）后0.5h，可以观察到小鼠胰岛中Ki67+细胞数目显著增加，小鼠胰岛的相对面积显著扩张，同时与β细胞增殖相关的蛋白（包括ERK、P38MAPK、P27、P21、CyclinD1、CyclinD3、Cdk4）的表达变化。
OBJECTIVE: To probe into the influence of CDK-5 on neurogeny and neural degeneration during cerebral development.
目的：探讨大脑发育过程中的周期素依赖性蛋白激酶5对神经系统的发生和退行性变的影响。
CDK and cyclin are key molecules that control and coordinate DNA-synthesis, chromosome separation and cell division.
CDK和细胞周期蛋白是控制和协调DNA合成、染色体分离和细胞分裂的重要分子。
CDK fully support Internet Explorer, FlashGet, NetAnts, Go!
周期素依赖性蛋白激酶完全支持Internet Explorer中，网际快车，网络蚂蚁，开始！
BMF could not be phosphorylated with Cyclin A-Cdk2 in a cell-free system.
BMF蛋白在无细胞体系中不能被CyclinA-Cdk2磷酸化。
The results suggested that Cdk5 promoted cell survival against cell death induced by serum deprivation.
提示在血清撤退细胞损伤模型中，Cdk5起着促进神经元存活的作用。
ERK pathway, which is also important in regulating cell survial, was not involved in μ opioid neuroprotection against cell death induced by serum deprivation.
5与细胞生存和死亡密切相关、并能间接调节Cdk5活性的ERK信号转导通路不参与μ阿片受体对血清撤离细胞损伤的保护作用。
Methods: Thirtyfive cholesteatomas tissues and twenty normal human external ear canal skin were used in this study.
采用免疫组织化学技术SABC法检测CDK4、P16在中耳胆脂瘤35例、正常外耳道皮肤20例中的表达，并结合胆脂瘤对听小骨骨质破坏程度，采用SPSS11.5软件进行统计学分析。
CDK activation by phosphorylation. In addition to cyclin binding, complete CDK activation requires phosphorylation at a conserved threonine residue(Thr 160 in CDK2). Phosphorylation may affect the cyclin binding site, as it enhances the binding of some CDK-cyclin pairs, conversely, cyclin binding may enhance phosphorylation.
CDK的完全活化，除了要与cyclin结合外，还需要的是一个保守的苏氨酸残基（theonine reside，Thr）磷酸化（人CDK2中Thr160），磷酸化可能影响cyclin结合位点，从而加强了CDK –cyclin复合物的结合，同样，cyclin结合可能加强了磷酸化，在正常脊椎动物细胞周期中，Thr160/161磷酸化的升高和降低与cyclin的结合状态趋于一种平行。3）去磷酸化抑制CDK活性。
At E10.5d, we separated the embryos of two groups, counted the radio of NTDs and the data of crown-rump partly, and used the immunohistochemical and flow cytometry to examine the expression of nestin, and with the methods of DNA quantity stain and RT-PCR to examine the transcription of Cdk2 and Cdk4s mRNA.
在孕期E10.5d时取出两组孕鼠的胚胎，分别计数胚胎的神经管畸形发生率及其顶臀距，应用免疫荧光组织化学染色和流式细胞仪检测胚胎神经管神经上皮的神经上皮干细胞蛋白（neuroepithelial stem cell protein，nestin）表达情况，应用DNA定量荧光染色和RT-PCR技术检测胚胎神经管依赖细胞周期蛋白激酶2（cyclin dependent protein kinase 2，Cdk2）mRNA和Cdk4 mRNA的转录。
Objective: To study the resistant function of QilingYiganjian to ascites hepatic carcinoma, influence to the expression of P21WAF1/CIP1 and CDK4, and the clinical study of QLYGJ to malignant tumor to discuss the possible mechanism of tumor resistance.
目的：研究芪灵益肝煎对小鼠肝癌的抑制作用及其对P21WAF1/CIP1(简称P21)和CDK4表达的影响以及芪灵益肝煎治疗恶性肿瘤的临床研究，以探讨该剂抗癌的可能机制。
As cells enter the cell cycle from quiescence, genes encoding D-type cyclins get activated at the beginning of the G1 phase.
当细胞从静止期进入细胞分裂时，编码D型cyclin的基因在G1期被激活，cyclin D随之与CDK4或CDK6形成一个复合物，活化后的激酶复合物磷酸化底物蛋白调控下游的通路。
Future studies may provide evidance for its potential for the treatment of tumor and metabolic diseases.
进一步研究CDK11P58的功能将为如肿瘤和代谢疾病的医治带来新的思路。
There was neither cyclin D1 nor cdk4 expression in chondroma of the jaws.
Cyclin D1和cdk4在颌骨软骨肉瘤过表达且与其发生和发展有关。
Objective To explore the expression and distribution of cyelin dependent kinase 11 （CDK11） after transected spinal cord injury.
目的探讨细胞周期蛋白依赖性蛋白激酶11（CDK11）在横断性脊髓损伤后的表达变化以及定位情况。
Expressions of yclin A, cyclin E and cdc2 were down-regulated; however, cyclin B1, cyclin D, CDK2, CDK4, and CDK6 werecup-regulated. About CDK inhibitor, p15, p16 and p21 expressions were enhanced In ZAK overexpressing H460 cells.
在细胞周期的部份我们观察到，当ZAK被过度表现后会使细胞周期停滞於S及G2/M期，细胞周期调控分子cyclin A、E及cdc2蛋白表现下降，cyclin B1、cyclin D、CDK2、CDK4及CDK6蛋白表现增加；CDK inhibitor的部份则是p15、p16及p21蛋白表现都有增强的情形。
The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-17 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN288 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN289 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-110 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T311 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.
应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。
METHODS CDK4 expression was detected by immunohistochemistry in 20 cases of chondrosarcoma, 8 cases of osteochondroma and 8 cases of hyperosteogeny of the jaws.
利用免疫组化法检测CDK4在20例颌骨软骨肉瘤、8例骨软骨瘤和8例骨质增生中的表达。
In this report, the molecular mechanism of cholesterol deficiency affecting proliferation of T lymphocytes was studied from the angle of cell cycle control.
近年来对细胞周期调控分子机制的研究已有突破性进展，使人们认识到细胞周期进程依赖于不同的cyclin／CDK复合物在恰当时间形成、激活及灭活［1］，其中cyclin是调节亚基，CDK是催化亚基。