AIM: To investigate the effect of purifried L-amino acid oxidase from bungarus fasciatus snake venom on apoptosis and growth of HUVCE cell line.
目的：探讨金环蛇毒中L-氨基酸氧化酶对人脐静脉内皮细胞株增殖和凋亡作用的影响。
Western blotting results showed that the expressed protein had similar immunogenicity with the natural α-BGT protein purified from the venom of Krait Bungarus spp..
经Western blot分析，在大约8 kDa处出现明显的目的带，与预计蛋白分子量大小一致，说明表达产物与天然α-银环蛇毒素具有相似的免疫原性。
Flow cytometry and laser confocal microscope showed a typical apoptotic peak and morphological changes of these cells. CONCLUSION: The L-amino acid oxidase from bungarus fasciatus snake venom could inhibit the HUEVC cell growth and induce the cell apoptosis.
通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，该氨基酸氧化酶是由单亚基组成的。L-氨基酸氧化酶能诱导HUVCE凋亡从而对其生长具有抑制作用。
The MTT assay was used to detect the viability of cells. Flow cytometry and laser confocal microscopy were used to identiyfy the cell cycle and apoptotic morphology after cells treated with LAO. RESULTS: An L-amino acid oxidase was successfully purified from the venom of bungarus fasciatus.
结果：通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，L－氨基酸氧化酶的表观分子量通过SDS-PAGE测定约为60 kD，通过凝胶过滤测定分子量为70 kD.L－氨基酸氧化酶作用于HUVCE细胞12 h后，细胞的生长明显受到抑制，L－氨基酸氧化酶作用的半数抑制量为2.8 mg/L。
Method8: The paPef, fOr the first time, studied the apoptosis or necrosis of K562cclls induced by bungarus multicinclus crude venom and several components frOmcrude venom by cation exchange chromatography'through the quatitative analysis offlow cytometry as well as the qualitative analysis, such as inversed opticalmicroscopy, MTT method and fluorescent microscopy, and so on.
本卖验采用羧甲基纤维素C-52阳离子交换层析将银环蛇粗毒进行了若干毒素组分的分离纯化，并以流式细胞术定量分析为主，以倒置显微镜、MTT比色法、荧光显微镜等多种定性分析为辅，首次对银环蛇粗毒及其若干组分蛋白对人慢性粒细胞白血病K562肿瘤细胞的细胞杀伤作用和凋亡诱导情况进行了研究。
The venom of elapid snakes contains a number of small proteins that display a broad spectrum of pharmacological activities. In study on the neurotoxin from the venom gland of Bungarus multicinctus, five cDNA sequences (MNCTL1-a, MNCTL1-b, MNCTL1-c, MNCTL1-d and MNCTL2) encoding two novel proteins were obtained from the total RNA by reverse transcription-polymerase chain reaction.
采用SMART技术和RT-PCR方法，从同一个银环蛇毒腺中克隆到了5 种尚未报道过的心脏毒素类似物全长cDNA序列（MNCTL1-a、MNCTL1-b、MNCTL1-c、MNCTL1-d和MNCTL2）。5种序列中，MNCTL1-b、MNCTL1-c、MNCTL1-d和MNCTL2的长度均为505 bp，包括5'非翻译区32 bp，3'非翻译区212 bp和其余261 bp组成的一个完整开放阅读框；MNCTL1-a 由于3'非翻译区24 bp的缺失，全长cDNA仅为481 bp。
The island is very rich in animal resources, monkeys (10 natural group of about 300), pangolins, civet cats, wild boar, Huang Xun, deer and other mammals; kite, Zhi, brown wings Arthur Juan, Sisheng cuckoo, seedling Chicken, Banjiu 40 species of birds, pythons, Bungarus fasciatus, Yinhuan snake, cobra, Trimeresurus stejnegeri, two snakes, Cuora trifasciata, grass lizards, such as Eumeces the more than 10 types of reptiles; rugulosa frog, Marsh frogs, Ze frog, Rana Ji, Tree Frog, black toad and other amphibians orbital; only insects are more than 40 kinds of butterflies.
岛上的动物资源也十分丰富，有猕猴(10个自然群约300只)、穿山甲、果子狸、野猪、黄勋、梅花鹿等哺乳类；鸢、鸷、褐翅鸦鹃、四声杜鹃、秧鸡、斑鸠等40多种鸟类；蟒蛇、金环蛇、银环蛇、眼镜蛇、竹叶青、两头蛇、三线闭壳龟、草蜥、石龙子等10余种处爬行类；虎纹蛙、沼蛙、泽蛙、姬蛙、树蛙、黑眶蟾蜍等两栖类；昆虫仅蝶类就有40多种。
Amphibians recorded were 8 species in 5 families, and reptiles were 13 species in 8 families. Among these, Guenther`s frog, Chinese cobra, and Taiwan banded krait are in the wildlife conservation species list of Taiwan.
其中陆栖哺乳类共5科11种，两生类5科8种，爬虫类8科13种，保育类野生动物有贡德氏赤蛙、眼镜蛇、雨伞节(Bungarus multicinctus multicinctus)等3种。
Objective To research the morphology difference of color variation Bungarus multicinctus and normal ones.
目的研究色变银环蛇与正常银环蛇的形态学差异。
This study confirmed that the venom of Bungarus fasciatus had anti-tumor cytotoxic effect, which would provide experimental basis for the clinical developing that anti-tumor agents of Bungarus fasciatus venom.
本研究证实了金环蛇蛇毒细胞毒素的抗肿瘤作用，为临床开发金环蛇蛇毒抗肿瘤制剂提供实验依据。
Results There are obviously difference between normal and color variation Bungarus multicinctus.
结果色变银环蛇与正常银环蛇的鳞片数目无异，但其毒腺大小、排毒量、毒性均有明显差异。
Advances of Research of Snake Venom from Bungarus Multicinctus and Genetic Engineering
银环蛇毒素及其基因工程研究进展
A binding characteristic of neurotoxins from Bungarus fasciatus venom to acetylcholine receptor
金环蛇神经毒素与乙酰胆碱受体结合的特点
According to the ecology and habit of Bungarus multicinctus multicincts, author had studied the reared technique of the animal for many years.
作者根据银环蛇的生态、生活等习性，对人工饲养技术进行了多年研究后证明：蛇园用植被绿化；
The results indicate that diagnostic PCR using the primers designed in the present study is a simple, effective and applicable method for the authentication of Bungarus parvus, and this method might also be a new way for examining the compositions of Chinese patent medicine.
研究结果表明用本鉴定引物对金钱白花蛇的PCR鉴定方法简便、有效，实用性强，该方法还有可能成为中成药复方组分鉴别的一种新手段。
Experimental Study on Anti-Inflammatory Effects of Composite Recipe of Bungarus Parvus
白花蛇复方抗炎作用的实验研究
Emergency nursing care of patients bitten by bungarus multicinctus
银环蛇咬伤病人的急救护理
Mechanical Ventilation for Four Patients with Acute Respiratory Failure by Bungarus multicinctus Blyth Snakebite
机械通气救治4例银环蛇咬伤急性呼吸衰竭
This paper reports the study on the difference of bile acid composition in the three kinds of snakes, i. e., Naja naja Linnaeus, Bungarus fascialus Schncider, and Ptyas Korros Schlegel.
本文着重研究两广产的三种蛇（金环蛇，眼镜蛇及过树龙）蛇胆中胆汁酸成分的差异。
Objective: To develop a convenient and effective method for the identification of Bungarus multicinctus.
目的：建立一种实用、简便的金钱白花蛇药材DNA分子标记鉴别方法。