Objective:YAG double-frequency laser microbeam was applied to study the effects on protein pattern of dimensional electrophoresis of tail bud embryos of Bufo bufo gargarizans.
目的：研究YAG倍频激光微束对中华大蟾蜍尾芽胚双向电泳蛋白质谱的影响。
The left lens of Anuran Tadpole - Bufo Raddei Strauch Tadpole and Xenopus laevis Tadpole were removed when they had posterior limb bud. By decapitating and fixing it with Carnoy's fixation fluid in different days, by means of paraffin serial section and Hematoxylin-Eosin stain and by using the microscope to observe the microstructure of lens regeneration of Bufo Raddei Strauch Tadpole and Xenopus laevis Tadpole. By extirpating the left eye and fixing it with aldehyde and osmium acid, by using electron microscope to observe the ultrastructure of lens regeneration of Bufo Raddei Strauch Tadpole and Xenopus laevis Tadpole.
以无尾两栖类—以花背蟾蜍与非洲爪蟾后肢芽期蝌蚪为实验材料，摘除其左眼晶状体，在再生不同时期固定，石蜡连续切片，苏木精-伊红对染，光镜观察，研究晶状体再生的组织来源；另外，选再生不同时期材料用戊二醛，锇酸固定，透射电镜观察，以研究晶状体再生组织的超微结构变化。
Blood oxygen capacity of Bufo bankorens is high compared with other anuran amphibians, which benefits their living at high altitude.
盘古蟾蜍血液携氧力比其他两栖类强，有助於生存在高海拔氧气较低的环境。
Effects of Laser Irradiation on Early-stage Embryonic Development of Bufo bufo gargarizans.
激光照射对中华大蟾蜍早期胚胎发育的影响
All results suggested that four herbicides can cause significant genetic damage s to the erythrocytes of Bufo bufo gargarizans.
这四种除草剂对蝌蚪红细胞具有显著的遗传毒性，其中使它隆的毒性要大于其它三种。
The translation activities of IL-2Ra mRNA among different kinds of cells by using Bufo bufo oocytes in vitro were studied.
本文采用中华大蟾蜍卵母细胞体外翻译系统研究了不同来源细胞IL—2RαmRNA的翻译活性。
Bufo marinus were kept for several weeks at 27℃.
縁蛙和豹蛙在5℃下保持若干星期。
The corneal development of Bufo raddei larvae during metamorphosis was studied through light and electron microscopy.
作者用光镜和电镜研究了花背蟾蜍蝌蚪变态期角膜的发育。
Effects of Laser Irradiation on Early-stage Embryonic Chromosomes of Bufo bufo gargarizans.
激光照射对中华大蟾蜍早期胚胎染色体的影响
Effects of Laser Irradiation on Early-stage Embryonic Isozymes of Bufo bufo gargarizans.
激光照射对中华大蟾蜍早期胚胎同工酶的影响
The research studied the gill in the Bufo raddei Strauch with the research object, used the methods of the paraffin section、semithin section、transmission electron microscope、Immuno-histochemistry, studied the change rule of the morphological structure and apoptosis in the gill development, discussed the relation of the apoptosis and the gill development.
本研究以花背蟾蜍发育过程中的鳃为研究对象，运用石蜡切片、半薄切片、透射电镜、免疫组化等相关技术，研究了花背蟾蜍鳃的发生，发育，退化过程中的形态结构及细胞凋亡的变化规律，探论了细胞凋亡与鳃发育之间的关系。
Bufotoxin collected from Bufo bufo garigarizans was diluted into 4 different kinds of concentration of 4, 6, 8 and 10mg/mL as a factor A, and Enterobacter aerogenes original solutions were diluted with LB liquid medium at 1∶104 and 1∶105 as a factor B.
把采自中华大蟾蜍的蟾酥用蒸馏水稀释成4、6、8、10mg/mL共4种浓度，作为因素A，用LB液体培养基将活化的产气肠杆菌原液按1∶104和1∶105稀释，作为因素B，采用二因子方差分析观察了蟾酥对产气肠杆菌抑菌圈直径的影响，并分析了蟾酥浓度与抑菌圈直径之间的相关关系。
The experiment was conducted to study the possible effects of toxicity in the toad erythrocyte s induced by dicofol, 48 mature toads, Bufo gargarizans, were injected with five different dosages of DCF in coelom every 24 h and 1-4 times in succession, and cells producing micronucleus, nuclear abnormality and cell abnormality were counted separately.
用5种不同剂量的三氯杀螨醇(d icofo l，DCF)分别对48只中华蟾蜍成体进行体腔注射法染毒1~4次，观察并统计中华蟾蜍成体外周血红细胞产生微核、核异常及细胞异常的细胞数。
21 days after taking brain, measurement of bufo single amine oxidase, superoxide dismutase (sod) and of mda. Results: 1.
21天后断头取脑，测单胺氧化酶、超氧化物歧化酶和丙二醛的含量。
The growth of Bufo melanostictus tadpoles in the water containing different concentrations of pesticide dichlorvos was studied using the static-renewal toxicity method.
采用静态换水法研究了杀虫剂敌敌畏(dichlorvos，DDVP)对黑眶蟾蜍蝌蚪的急性毒性和生长发育、生理活动的影响。
Blastomeres of the two, four and eight cell stage as well as embryos of the gastrula stage of Bufo melanostictus were irradiated with ruby laser microbeam of focusing diameter 10μ, and pulse duration lusec.
正在分裂的卵被照射后，发育成为各样的畸形胚胎或蝌蚪而死亡，只少数能够发育至变态成小蟾蜍。
Isolation and identification of bufogenin constituents from the skin of Bufo bufo gargarizans Cantor
蟾皮中蟾毒配基类成分的分离与鉴定
Ultraviolet irradiation influenced on embryo's development of Bufo bufo.
紫外线照射可影响蟾蛤类的胚胎发育。
Shock complicated by disseminated intravascular coagulation (DIC) in rabbits and dogs and endoxemia in rats were induced by intravenous injection of endotoxin (LD_ (75)) from E. coli to compare the antishock effect between anisodamine (AA) and bufo extract (BE).
给动物体内注入内毒素（ID（75））引起兔和狗实验性休克合并DIC，以及大鼠内毒素血症后，比较了山莨菪碱（AA）和蟾酥（BE）抗休克的作用。
In order to investigate the mechanism of inhibitory effect of tyrosine on HCG, the effect of tyrosine on HCG induced membrane potential depolarization was observed in vitro by means of recording membrane potential of toad (Bufo bufo gargarizans) oocytes using microelectrodes.
为深入探讨酪氨酸抗HCG作用的机理，本文利用微电极细胞内记录的方法，观察了HCG、酪氨酸培养下卵母细胞膜电位的变化。