It was found that modified PTI became sensitized to subtilisin, and the STI of DSP can be hydrolyzed by subtilisin. The K_m, and V_ value of subtilisin for PTI and STI were 657.9BAEE/mL and 606.1BAEE/mL, 104.9BAEE/ and 106.6BAEE/ respectively.
研究结果表明：酶水解花生胰蛋白酶抑制子的最适反应条件为：反应温度40～60℃、pH值7.0～8.0、酶活力≥40 000IU／mL以及≥60min的反应时间；水解大豆胰蛋白酶抑制子的最适反应条件为：反应温度35～55℃、pH值7.5～8.5、酶活力≥40 000IU／mL以及≥40min的反应时间。
Methods The sperm acrosin activity was examined by BAEE/ADH joint method.
方法精子顶体酶活力检测采用BAEEADH联合法。
Methods: Sperm acrosin activity was measured by BAEE/ADH and AR evaluated by FITC-PSA.
方法：采用BAEE/ADH法测定精子顶体酶的活性，以及通过FITC-PSA法检测精子顶体反应。
They all belonged to trypsin-like serine proteinase family. They were glycoproteins, and could hydrolyze casein, fibrin and BAEE.
组分8、9、10都是有类胰蛋白酶活性的丝氨酸蛋白酶，都是糖蛋白，都能水解酪蛋白、纤维蛋白和BAEE。
Na +, K +, Mg2 +, Hg2 +, EDTA and mercaptoethanol show some inhibition with various degrees. Ca2 + can increase its activity.
该酶水解BAEE的活力可被Na＋、K＋、Mg2＋、Hg2＋、金属离子和EDTA、巯基乙醇抑制，Ca＋则有激活作用。
On the basis of enzymatic activities against BAEE and various inhibition, F6 was a serine protease and metalloprotease, and the other five enzymes were trypsin-like serine proteases.
水解BAEE试验及纤溶活性抑制试验表明，F6既是丝氨酸蛋白酶，又是含金属离子的蛋白酶，其它5种酶为胰蛋白酶样的丝氨酸蛋白酶；
Results indicated that the modified PPK showed an increase in molecular weight, tolerance to temperature and stability, and a decrease in BAEE activity.
结果表明，修饰PPK分子质量增加，保留活性降低，对温度的耐受性加强，稳定性增加。
Methods The sperm acrosin activity was tested by the method of BAEE/ADH Unity, andacrosome reaction by the Triple-stain technique.
方法采用BAEE/ADH联合法测定顶体酶和三色染色法技术测定顶体反应。
The experiment had proved that the enzyme could not only hydrolyze casein but also hydrolyze BAEE.
实验证明，该酶既能水解酪蛋白，又能水解N-苯甲酞-L精氨酸乙酯（BAEE）。
In this paper, the writers improve greatly the method of determinating enzyme activity with synthetized substrate & BAEE.
本文在全面测定了温度，底物浓度、酶浓度和酶促反应时间等条件的基础上，对用合成底物BAEE测定酶活性的方法作了较大改进。