In this study, we detected 56 patients among Chinese population of -α3.7 defect in alpha globin gene by PCR method, then the PCR product was digested by the restriction enzyme ApalⅠand BalⅠ.
本研究在中国人群中用PCR基因分析方法检出具有α珠蛋白基因-α3.7缺失的患者56例，然后用ApalⅠ和BalⅠ限制性内切酶进行分型。
In this paper, full sequence of porcine growth hormone gene was amplified in Large Yorkshire and Landrace by PCR. The amplification products were analyzed in sequence polymorphisms. BstXI, Apal, Hhal and Mspl polymorphisms were detected. Effects of different genotypes on some important production performance were analyzed according to the acquired data.
本试验以大约克猪和长白猪为研究对象，利用PCR技术扩增了猪生长激素基因全序列，并对PCR产物进行了序列分析，以及对BstⅪ、ApaⅠ、HhaⅠ和MspⅠ 4种内切酶酶切位点多态性进行了研究，同时结合性能测定资料，分析了不同基因型或带型与生产性状的关系。
Two SNPs, a Taq Ⅰ in exon Ⅰ and an ApaL Ⅰ in intron Ⅲ, were found within chicken A-FABP gene in F〓 population and divergent lines.
8在鸡I-FABP基因的内含子Ⅱ和内含子Ⅰ中发现两个多态性位点，分别是内含子Ⅱ中的SnaBⅠ酶切多态位点和内含子Ⅰ中的PvuⅡ酶切多态位点。I-FABP基因的多态性与鸡的体重显著或极显著相关（P＜0.05或P＜0.01）。
No plasmid was extracted and conjugation was not successful, despite multiple attempts. Southern blot confirmed that the armA gene was located on the chromosome Apal digested DNA patterns of these strains.
碱裂解法反复抽提未得到质粒，多次接合试验未成功；Southern杂交显示armA基因分别位于克隆A、B、C菌株染色体ApaI酶切片段约220kb、300kb、220kb大小的片段上。