Methods: Adult mice of Kunming variety were used as experiment animals. After judgement of oestrus, the oestrus period and oestrus stage were determined, and then those mouse were grouped depending on oestrus stage. The mice were dealed with diferrent dosage of PMSG and HCG to make them superovulation, afterwords were mated individually with the male mouse together at same cage. The embryoes were rinsed out and evaluated after 3.5 days when the plug had been seen. The transplant mice were born after the high-quality embryoes were transferred into the receptors mouse uterus at the same oestrus stage which was stimulated by mating with the male mouse which spermaduct had been ligated previously.
以昆明系小白鼠为实验动物，取性成熟个体，先进行发情鉴定，确定其发情周期及不同的阶段，然后按发情阶段分组，分别用不同剂量的孕马血清促性腺激素和人绒毛膜促性腺激素进行超排处理并与公鼠合笼，在见栓后的第3.5天冲胚，对所得胚胎进行鉴定；将质量较好的胚胎选出，移入同期发情并经结扎公鼠交配刺激后见栓的受体小鼠子宫内，得到移植小鼠。
This experiment selects material E14d SD the big mouse SVZ nerve stemcell as well as P7d the SD big mouse internal ear stem cell, does nothave the blood serum complete culture medium raise through D-MEM/F12to cause its massive multiplication, a part carries on Nestin, NF, CNP, GFAP, MAP-2, Myosin, the P27 immunity group, proved the raise thenerve stem cell has many to the split up ON potential; Another partdyes EGFP after the extension to carry on the guinea pig internal earto transplant, after two weeks fills the class to take the cochleafixed slice observation, discovered has the stem cell ball group inthe internal ear survival, proved the nerve stem cellheterotransplantation the possibility and for transplants the stemcell migration differentiation repair hair cell damage the thoroughresearch accumulation experiment experience.
本实验取材E14d SD大鼠SVZ神经干细胞以及P7d SD大鼠内耳干细胞，通过D-MEM/F12无血清完全培养基培养使其大量增殖，一部分进行Nestin、NF、CNP、GFAP、MAP-2、Myosin、P27免疫组化，证明培养的神经干细胞具有多向分化的潜能；另一部分通过转染EGFP后进行豚鼠内耳移植，两周后灌流取耳蜗固定切片观察，发现有干细胞球团在内耳存活，证明神经干细胞异体移植的可能性并为移植干细胞迁移分化修复毛细胞损伤的深入研究积累实验经验。
According to all-around investigating on wild Lonicera fragrantissima in various mountainous areas of Henan Province, this article has maken clear Lonicera fragrantissima regional distribution, Total resources quantity, biologic characteristics, ecologic characteristics, natural distribution patterns, the value of development and utilization, using form, cuttage and breeding technology, the collection and protection of wild idioplasmic resources and so on. It detailedly describes wild resources distribution, biologic characteristics and ecologic characteristics. It has classified and selected a batch of superior trees according to the differences in fruits shape, fruits color, leaf shape and shape of the crown of trees. It has based on the nutrition and medication ingredient analyzed the using methods of fruits and leaves, and the way of exploitation. Through the experiment on Lonicera fragrantissima cuttage and breeding technology, transplant and domestication technology, it has provided the concrete technical measure of idioplasmic resources use and the preservation for Lonicera fragrantissima.
本文通过对河南省各山区野生郁香忍冬资源的全面调查与研究，摸清全省郁香忍冬资源的区域分布、资源总量、生物学特性、生态学特性、自然类型与经济性状、郁香忍冬的开发利用价值、利用形式、扦插育苗技术和野生种质资源收集保存与保护方法措施等，对河南省郁香忍冬野生资源的分布，生物学特性、生态学特性进行详细描述总结；根据果形、果色、叶形、冠形的差异性进行了类型划分，并选出了一批优树；根据果实、叶片中的营养与药用成分分析、果、叶利用方法研究，分析了其开发利用途径，并开展了郁香忍冬扦插育苗试验和苗木移栽训化试验，为郁香忍冬种质资源利用和保存提供了具体技术措施，其主要结果如下：1、郁香忍冬属中性偏阳的低矮灌木树种，在灌木坡上当其处于上层空间时或纯林分布时，生长最好。
There are significant statistical differences. (P0.05). Terminate the experiment after the gross anatomy and histological observation were not found obvious with the new artificial blood vessel transplant-related organ damage and organizational structure changes, and no thrombosis.
（P0.05）。终止实验后的大体解剖和组织切片观察均未发现明显的与新型人造血管移植后相关的器官损伤和组织结构变化，未见血栓形成。
Using surgical knife to cut eggshell round, plastics bag to transplant chicken embryo and concave eggshell and paraffin wax to seal airtight, the experiment studied two simple methods of in vitro culture for X-period chicken embryo.
以原蛋壳为孵化蛋壳，利用塑料袋对鸡胚进行转壳，用保鲜膜或蛋壳皿和石蜡封口，分别对I、II两种简易方法的X期鸡胚体外培养进行了研究。
Through orthogonal experiment design, coconut hust and peat were taken as light media in experiment of Eucalyptus tissue-culture shoots transplant.
通过正交试验设计，以椰糠和泥炭土作为轻型基质进行桉树组培苗移栽育苗试验。
X-ray Expression and pathological Observation of the Animal Experiment on the Treatment of Avascular Necrosis of the Femoral Head with the pedicled Greater Omentum Transplant
带蒂大网膜移植治疗股骨头缺血性坏死&动物实验的X线及病理观察
Using field comparative experiment of transplant rice seedlings to research the result of transplanter which model is2ZT-9356 (B).
利用水稻田间插秧对比试验，研究2ZT-9356（B）型机动插秧机作业效果。