Endostatin was amplified from Pshuttle-Endostatin plasmid with PCR and subcloned into the pAdTrack-CMV shuttle vector after sequencing and identification. The resultant plasmid was co-transduced into E. coli BJ 5183 cells with pAdEasy-1 plasmid for homologous recombination. The recombinant plasmid was detected by restriction analysis and DNA sequence analysis. The linearized recombinant plasmid was transfected into AAV 293 cells with LipofectAMINE2000. The recombinant adenovirus was detected by GFP, PCR and restriction analysis. The virus was used to infect ECV304, and the expression of endostatin in vitro was detected by the techniques of RT-PCR and Western blot.
以Pshuttle-Endostatin质粒为模板，应用特异引物，通过聚合酶链反应扩增纯化得到Endostatin基因片断，经测序鉴定后，酶切插入穿梭质粒pAdTrack-CMV中，再与骨架质粒AdEasy-1在大肠杆菌BJ5183中进行同源重组，重组质粒经筛选、提取、纯化后，经酶切及测序鉴定正确，再大量扩增为腺病毒载体，线性化后利用脂质体2000转染AAV293细胞包装并扩增，得到的病毒液纯化扩增后，感染人脐静脉内皮细胞，反转录-聚合酶链反应检测感染细胞中目的基因的表达，蛋白免疫印迹检测感染细胞中蛋白的表达。
A In the space below, draw a vector diagram to show the resultant velocity of the ball.
a 在下面的坐标图上画出球的合速度，并清楚地标明所有速度矢量。
Based on customer lifetime value of marketing database to determine the effect of the evaluation system so as to the theoretical basis for fuzzy math, fuzzy comprehensive evaluation of the use of secondary theory, build a secondary fuzzy comprehensive evaluation model, the adoption of the final composite computing has been the single factor evaluation As a result, while these factors and the evaluation of the weight vector matrix composite computing, the resultant effect of the enterprise database marketin
所以影响顾客终生价值大小的若干因素对数据库营销效果的影响显而易见。本文通过对顾客终生价值的探讨来确定数据库营销效果的评价指标体系，以模糊数学为理论基础，运用二级模糊综合评价理论，构建二级模糊综合评价模型，最后通过复合运算得到各单因素评价结果，同时对这些因素进行权重向量与评价矩阵的复合运算，由此得到企业数据库营销效果的评价结果。最后选取某超市的顾客信息对上一章的模型进行实证检验，并总结了这个论文的研究内容，提出文章的创新点，找出问题和不足，以及以后进一步的研究方向。
The method of feasible directions based on vector resultant
基于矢量合成的可行方向法
To further investigate the effect of H-NS on APP biofilm formation, the 408 bp complete coding sequence of hns gene was amplified by PCR from the genomic DNA of APP serotype 1 strain 4074 and cloned into the prokaryotic expression vector pET-28c. The resultant recombinant plasmid pET-hns was transformed into Escherichia coli BL21 (DE3). A 19KDa of recombinant protein was obtained by IPTG induction, and purified using the Ni-NTA agarose columns.
为了进一步研究H-NS蛋白对APP生物被膜形成的影响，本文以APP血清1型4074株基因组DNA为模板，PCR扩增了408bp的hns基因编码区，克隆到原核表达载体pET-28c中获得重组质粒pET-hns，转化大肠杆菌BL21(DE3)，经IPTG诱导表达和组氨酸亲和层析柱纯化获得大小约19KDa的重组蛋白rH-NS。
Term for the formula true: the system is out of external force or the resultant vector of the external force received is zero.
这个式子成立的条件，是系统不受外力或受到的外力的矢量和为零。