By push-pull perfusion technique and radioenzymaticassay, it was found that after 10 min of acupuncture, pain threshold increased significantly, while the content of NA in the perfusate from the preoptic area was markedly decreased.
Push-pull perfusion technique, radioenzymaticassay and radioimmunoassay were used to observe the changes of noradrenaline and leueine-enkephalin release from brain nuclei and dorsal horn of spinal cord in rabbits.