Immunohistofluorescent staining was used to identify the binding specificity of GEBP11 in gastric cancer tissues. 5. GEBP11 was labeled with ~(99)Tc~mO_4~- using direct labeling method, and was labeled with ~(131)I using NBS labeling method. Then the labeled peptides were validated for radiochemical yield, specific activity, and in vitro. stability. 6. The bioactivity of ~(99)Tc~m-GEBP11 or ~(131)I-GEBP11 was validated by cell receptor autoradiography in cultured HUVECs. 7. Receptor binding assay was performed in vitro. to analyze quantitatively the binding specificity and affinity of GEBP11 to HUVEC, the receptor intensity on HUVEC, and the difference of affinity of GEBP11 binding to Co-HUVEC and HUVEC. 8. The radioactivity of all organs of nude mice injected with ~(131)I-GEBP11 was determined and the radioactivity of all organs per g was calculated to analyze the specific distribution of ~(131)I-GEBP11.9. Gamma camera images of nude mice bearing tumor xenografts of human gastric carcinoma injected with ~(99)Tc~m-GEBP11 was obtained 0.5-24 h after being injected to identify the targeting ability of labeled peptide to tumor tissues. 10. MTT assay on HUVECs and antitumor assay in nude mice bearing tumor xenografts of human gastric cancer were performed to evaluate the effects of ~(131)I-GEBP11 on HUVECs and xenografts. 11. Immunohistochemical staining was used for MVD counting in tumor. 12. H. E. staining, blood cells analysis and blood biochemical indicator analysis were performed to investigate the injury of liver and bone marrow. 13. Tube formation assay in matrigel, CAM angiogenesis assay and angionenesis induced by matrigel in mice were performed to identify the effects of GEBP11 on angiogenesis.
1、通过蓝色噬斑形成实验及GEBP11、IN11噬菌体在荷瘤裸鼠体内的竞争抑制实验检测噬菌体体内归巢的特异性；2、免疫荧光技术检测GEBP11在荷瘤裸鼠体内的结合特异性；3、免疫细胞化学或荧光检测GEBP11在Co-HUVECs中的定位及内化；4、免疫组织荧光检测GEBP11在人胃癌组织中的结合特异性；5、采用直接法~(99)Tc~mO_4~-标记GEBP11，采用NBS法~(131)I标记GEBP11，纸层析法测定标记率、放射化学纯度等；6、放射自显影鉴定GEBP11同位素探针的结合活性；7、受体放射配基结合分析实验鉴定受体亲和力及受体细胞密度；8、同位素示踪技术检测~(131)I-GEBP11在荷瘤裸鼠体内的生物学分布；9、SPECT成像技术进行荷人胃癌裸鼠体内~(99)Tc~m-GEBP11显像；10、MTT细胞增殖实验、荷瘤鼠抑瘤实验评价~(131)I-GEBP11对内皮细胞及荷瘤鼠肿瘤的抑制能力；11、免疫组织化学染色计数瘤组织MVD；12、病理学HE染色、血液血细胞分析及生化指标检测观察肝脏损伤及骨髓抑制情况；13、Matrigel管状结构形成实验、鸡胚绒毛尿囊膜血管生成抑制实验、小鼠体内Matrigel Plug诱导血管生成实验分析GEBP11对血管生成的影响；14、MTT细胞增殖实验、细胞周期及凋亡分析、细胞侵袭迁移实验、细胞粘附实验探讨GEBP11抑制血管生成的细胞机制；15、基因表达谱芯片技术筛选GEBP11短肽作用Co-HUVECs后的差异表达基因。
Determined the affinity and selectivity of all the analogues to μ and δ opioid receptors in the rat brain P_2 membrane by radio ligand competition experiment. Their activity on GPI and MVD preparation were assayed. Antinociceptive activity on mouse hot-plate test and degradation properties on mouse brain homogenate of some analogues were tested.
通过放射配体竞争结合实验测定了以上化合物对大鼠脑膜上μ和6阿片受体的亲和性和选择性；通过离体器官生物检定实验测定了它们在豚鼠回肠纵行肌和小鼠输精管上的活性；选择有代表性的化合物测定了小鼠热板模型上的镇痛活性和小鼠脑匀浆降解特性。
MVD was counted for CD34 positive vessels. Results: CD34 was extensive positive expressed in AB. In malignant AB, vessels appearedin catenuliform around invasive tumor cells or were sleeved by tumor cells. In benign AB, vessels were extensive scattered or appeared in cluster around invasive tumor cell.
结果 在AB中CD34呈广泛阳性表达，血管分布有三种形式，恶性AB多为以血管为中心肿瘤细胞呈袖套状围绕其周围或在侵袭性瘤细胞周缘血管呈串珠状排列，良性AB血管多为弥漫散在或在侵袭性肿瘤周边呈簇状排列。
Results In the groups of non-lymphaden metastasis and lymphaden metastasis, the positive rates of CD34 protein were 55.00%(11/20) and 40.00%(4/10)respectively and the MDV were 26±6 and 24±5 respectively.
结果CD34蛋白在膀胱移行细胞癌无淋巴结转移组和淋巴结转移组中的阳性表达率分别为55.00%和40.00%，MVD值分别为26±6，24±5。
The MVD and MVA of the hippocampus and frontal cortex in aged rats were markedly lower than those in young rats.
老龄大鼠海马及额叶皮质的MVD值、MVA值均明显低于青龄大鼠，这是老年血管性痴呆发生机制的主要形态学依据。
To evaluate the effect of Octreotid on the expression of MVD and VEGF in seeding carcinoma.
目的 探讨奥曲肽对肝癌微血管密度和血管内皮生长因子表达的影响。
Methtods 29 patients with HCC was examind by GE voluson 730 three-dimensional color ultrasound system, VI、FI and VFI was measured by using three-dimensional color histogram technique and MVD was measured postoperation.
应用GE voluson 730三维彩色超声仪对29例肝细胞癌进行检查，采用三维彩色直方图技术定量测量血管指数（Vascularization index，VI）、血流指数（Flow index，FI）和血管血流指数（Vascularization-flow，VFI），术后测定标本的肿瘤微血管密度(microvessel density，MVD)。
Series transmitters and high MVD NOC quasi-ELITE, F Series, H series, T series and R series Coriolis meter is compatible with two-phase, three-phase separator or a compact with the use, including the GLCC (cylindrical rotation current gas-liquid separator).
3000系列 MVD NOC 变送器与高准 ELITE、F 系列、H 系列、T 系列以及 R 系列科里奥利仪表兼容，可与两相、三相或紧凑型分离器配合使用，包括 GLCC。
Results The expression of tenascin and MVD varied with tumor cell differentiation and tumor stage, the differences were significant.
结果不同病理级别的胶质瘤胞浆内Tenascin表达程度和MVD差异明显，Tenascin表达和MVD有相关性(P.01)。
Objective To investigate the relationship between PTEN and microvessel density, and to explore its significance in human astrocytomas.
目的 探讨人脑星形胶质细胞瘤中PTEN表达与微血管密度(microvessel density，MVD)之间的关系及意义。
The grid number of flow signal's pixel was measured with the computer, and its result was compared with MVD.
计算机辅助测定超声造影前后血流信号像素网格数，并与微血管密度对照分析。
Objective To study the expression of epithelial-cadherin in breast cancer cells and the microvessel density in breast cancer mesenchyma, and to analyze the correlation between two factors and metastasis in breast cancer.
目的：研究乳腺癌细胞上表皮细胞黏附分子的表达和癌间质的微血管密度，并分析E-cd和MVD与乳腺癌转移的关系。
MVD and VEGF were the main pathological factors of DCT of adrenal masses, which resulted in different T-D curve, Washr and Washa of adrenal DCT.
MVD和VEGF可能是导致腺瘤和非腺瘤具有不同的T-D曲线类型和廓清率的主要因素之一。
Adenomas were suggested when adrenal masses presented as Type A and C, and/or Washr≥34%, and/or Washa≥43%, vice versa, nonadenoma. These findings showed close correlation of both enhancement appearances of DCT and MVD and VEGF expression of adrenal masses.
肿块廓清曲线为A、C型，和Washr≥34 HU，和Washa≥43%组均提示为腺瘤，反之提示为非腺瘤。T-D曲线廓清迅速组、7 min延时点Washr≥34%组和Washa≥43%组MVD、VEGF表达水平分别高于廓清缓慢组、＜34%组和＜43%组；由此提示动态增强CT表现特征与MVD、VEGF表达存在相关性。
Methods A total of 458 patients with idiopathic trigeminal neuralgia treated by MVD from January 2002 to August 2008 were analyzed retrospectively.
回顾复习微血管减压手术458例，对手术技术及责任血管的处理方法进行改良。
Results The value of MVD with marked CD105 was 36.50±9.43 in colorectal cancer.
结果 60例结直肠癌中CD105表达的MVD为36.50±9.43。
Part 1 PTEN Expression in Bladder Cancer and Its Relationship to BloodVessel Productions1、PTEN Expression in Bladder Cancer and the Relationship between PTEN and Microvessel DensityObjective PTEN (phosphatase and tensin homologue deleted from chromosome 10) is the first anti-oncogene being a phosphatase, and its expression correlates with tumor development and prognosis.
第一部分膀胱移行细胞癌组织中PTEN表达及其与血管生成的关系一、膀胱移行细胞癌组织中PTEN的表达及其与MVD的相关性目的PTEN是迄今为止发现的第一个具有磷酸酶活性的抑癌基因，其表达与肿瘤的进程和预后相关。
However, the value of SCT for detecting mucous layer invasion and proper layer invasion is limited.(2) The expression of MVD and PCNA is a biological label of malignancy.
1。良好的扫描前准备和扫描方法是螺旋CT对膀胱癌正确分期诊断的关键，尤其对膀胱壁外侵犯的诊断具有明显优势，但是对于判断粘膜层及固有层浸润的诊断仍有待于进一步提高。2。
Being a kind of important acyclic degrees, β acyclic has become basic demand of normalization with lossless join, keep dependency and 4NF under MVD environment, and can guide directly database schema design.
无β环是一种重要的无环级别，与无损联接、保持依赖和4NF一起，成为多值依赖环境下数据库模式规范化的基本要求，能够直接指导数据库的模式设计。
Therefore, MVD is a potential technology for drying Pteridium aquilinum.
微波真空干燥技术是适合蕨菜脱水的有潜力的干燥技术。